Shubhankar Das
Cambridge,SHF
Summary
A diligent and detail-oriented scientist with practical knowledge in molecular biology, cell culture, and analytical methods. Strong experience with laboratory operations, including cell-based assays, primary cell isolation, and working with biologic. Proficient in maintaining high research standards, data integrity, and adherence to SOPs and safety requirements. Experienced in debugging experiments, working in cross-functional teams, and presenting scientific findings. I'm passionate in contributing to cutting-edge immunology and biotherapeutics research.
Overview
9
9
years of professional experience
1
1
Certification
Work History
FOH
Zizzi
Sheffield
09.2023 - Current
- Delivered great customer service in a fast-paced restaurant setting, ensuring that guests had a positive eating experience.
- Efficiently managed orders, collaborated with kitchen personnel, and handled customer inquiries.
- Maintaining cleanliness and organization in dining rooms while following health and safety rules.
Student Ambassador
University of Sheffield
Sheffield
02.2023 - 07.2023
- Engaged with prospective students, providing information and delivering presentations about university programs, enhancing communication and interpersonal skills.
- Assisted in organizing open days, campus tours, and outreach events, demonstrating strong teamwork and organizational abilities.
- Adapted to dynamic situations, addressing student concerns and providing on-the-spot solutions, showcasing problem-solving skills.
Laboratory Assistant
SRL Diagnostics
07.2021 - 07.2022
- Conducted microbiological testing and analytical processes while making sure that SOPs and quality requirements were followed.
- Maintained and prepared solutions, reagents, and working cultures to support laboratory operations.
- Helped with the calibration, troubleshooting, and maintenance of the equipment to guarantee accurate and trustworthy test findings.
- Maintained a clean and safe working environment by keeping an eye on consumables, managing lab inventories, and keeping an eye on cleanliness.
INSPIRE Intern
Department of Science and Technology
07.2016 - 08.2016
- Assisted with laboratory maintenance, such as managing supplies, creating analytical solutions, and keeping the workstation clean and orderly.
- Supported senior researchers during experimental procedures, exhibiting meticulous attention to detail and adherence to laboratory protocols.
- Gained hands-on expertise with lab equipment maintenance and troubleshooting while adhering to safety and quality requirements.
Education
MSc - Molecular Biology and Biotechnology
UNIVERSITY OF SHEFFIELD
Sheffield, United Kingdom01-2024
BSc(Hons) - Biotechnology
GURU GHASIDAS UNIVERSITY
India01.2021
Skills
- DNA Extraction
- RNA extraction
- PCR
- Rt PCR
- QPCR
- Gel electrophoresis
- Protein Extraction & analysis(SDS & Western Blotting)
- Microscopy
- Column Chromatography
- CRISPR Cas9
- Cell culture (bacteria & yeast)
- Bradford Assay
- Plasmid Design and Construction
- Genomics
- DNA sequencing
- ELISA
- Flow Cytometry
- SOPs (Report Writing)
- Statistical Analysis
- Instrument Handling
- Effective Communication
- Critical Thinking
- Flexibility
- Time management & organizational
- Leadership
- Microsoft Office(Word Excel PowerPoint)
- R programming
- Snapgene
- Graphpad
- Image J
- FIJI
Certification
- IMUNA participation certificate, International Model United Nations, 01/2021
- INSPIRE Internship Programme, Department of Science and Technology India, 07/2016
- Fundamentals of Flow Cytometry Course, BIORAD, 2024
References
References available upon request.
ENHANCING PHOTOSYNTHETIC EFFICIENCY IN GM RICE
The overexpression of Fructose bis Phosphate Aldolase in the C3 cycle was conducted to enhance carbon flux within the rice genome, the gene was introduced into the rice genome through Agrobacterium-mediated transformation.
DNA and RNA extraction to isolate genetic material from transformed plants, and gel electrophoresis to analyze the samples; polymerase chain reaction (PCR) and real-time PCR (qPCR) were utilized for FBPA gene amplification and quantification of gene expression; sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) was used for protein separation, followed by immunoblotting to detect the FBPA expression
Phenotypic analysis was carried out to assess the physiological characteristics of the plants, including measurements of plant height and gross weight
KNOCKING OUT THE MET15 GENE IN SACCHAROMYCES CEREVISIAE USING THE CRISPR/CAS9 SYSTEM AND INSERTION OF NATMX GENE CASSETTE IN THE MET15
Implemented cutting-edge CRISPR-Cas9 technology for precise genetic modifications, utilized Cas9 and sgRNA to disrupt the Met15 gene, inducing targeted double-strand cleavage, and facilitating repair through NHEJ, successfully integrated NatMx gene by co-transforming yeast with pBH750 and pre-cut pMSc-MBB (NatMx), confirmed accurate gene integration using PCR techniques, validated gene integration through Western Blot analysis of NatMx-Myc9 expression, and contributed to advancing genetic engineering capabilities for yeast strains.
References
References available upon request.
Timeline
FOH
Zizzi
09.2023 - Current
Student Ambassador
University of Sheffield
02.2023 - 07.2023
Laboratory Assistant
SRL Diagnostics
07.2021 - 07.2022
INSPIRE Intern
Department of Science and Technology
07.2016 - 08.2016
MSc - Molecular Biology and Biotechnology
UNIVERSITY OF SHEFFIELD
BSc(Hons) - Biotechnology
GURU GHASIDAS UNIVERSITY
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