Ammar Mayah

A motivated, adaptable, flexible, determined, committed and responsible Cell Scientist at Genomic Instability and Cell Communication Research Group/ Oxford Brookes University seeks a Cell Scientist position who will use the professional and technical skills developed through approximately three decades in the scientific research field, particularly in cell biology research and cell functional assays based on tissue culture, cell biology molecular biology, flow cytometry technique, ELISA, cell-cell communication, bioimaging, immunohistochemistry-immunocytochemistry, western blotting, EVs/exosome and exosomal nucleic acid and protein molecule research.

I led the research team as a Research and Development Lead at OTT, I have extensive experience in daily flow cytometry techniques, including measuring cell toxicity, viability, proliferation, cell cycle, and detecting target proteins. I am eager to bring my expertise to your team and contribute to advancing your research objectives in flow cytometry. Flow cytometry has become essential for measuring daily data across various biological processes in our laboratory. I usually use flow cytometry to assess critical parameters such as cell cycle progression, cell toxicity levels targeting CDC20, Cyclin B, Securin and other cell mitotic proteins, proliferation rates, apoptosis induction, DNA damage extent, and the detection of specific targeted proteins within our samples.

I have also, collaborated with esteemed professionals like Dr. Riyadh Alakeely at Oxford Expression Technologies, I have acquired invaluable expertise in Co-transfection and ELISA techniques, particularly in the context of diagnosing Crimean-Congo haemorrhagic fever (CCHF).

DNA, RNA and PCR techniques have been extensively performed daily in our lab. I extracted DNA, RNA and microRNA and amplified them using PCR and RT-qPCR (please see my publications). I also used Droplet Digital PCR (ddPCR), but it was for a few times. Protein molecules from cells, EVs and exosomes are also extracted and measured in our lab.

Hence, I chaired a “Non-coding RNA and radiation responses” linked to the exosome session in ICRR 2019 in Manchester.

Currently, I am a Visiting Researcher at Genomic Instability and Cell Communication Research Group, Biological and Medical Sciences at Oxford Brookes University. We investigate the answer lies in cell communication, an ability to create, transmit, receive and respond to chemical and electrical signals. Advancing our understanding of cell communication is essential to many biological and pharmaceutical fields. I've successfully developed lab protocols and techniques related to surface exosomal RNA extraction and characterisation from culture media, blood plasma, and body organs, for which a project has been submitted for validation.

In brief, Highly skilled professional with extensive experience in tissue culture, bioimaging techniques, and flow cytometry. Demonstrated expertise in cell cytotoxicity and viability assays, exosome extraction and characterisation, and DNA/RNA extraction and PCR techniques. Proficient in measuring DNA damage, co-transfection using recombinant baculovirus, protein extraction and purification, ELISA analysis, western blotting, immunohistochemistry assays, telomerase activity measurement (TRAP assay), telomere length measurement, mycoplasma testing and bacteria cultivation. Experienced in cell karyotyping using advanced techniques such as M-FISH and G-banding. Notable achievements include developing an indirect ELISA kit for rapid diagnosis of Crimean-Congo Haemorrhagic Fever virus with high sensitivity and specificity. Career goal: to leverage extensive laboratory skills to contribute to innovative research in cellular biology.

I supervised, trained and mentored postdoctoral researchers, PhD students, technicians and eight undergraduates (every year since 2016) on their final-year projects. Additionally, I was also involved in a PhD study, “Metastatic cell responses to anticancer therapy: The possible role of cancer stem cells” December 2018.

• Novel micromolecules-induced cell cycle inhibition in breast cancer cells. February 2024.
• Metastatic cell responses to anticancer therapy: The possible role of cancer stem cells. December 2018.
• Investigating the Possibility of Genetic Resistance or Susceptibility in X-Irradiation Induced Senescence. September 2017.
• The effects of ionising radiation on cell senescence and DNA damage in HF19 lung cells. September 2016.
• Exosomes from ICCM-mediated DNA damage in human epithelial prostate cancer cells. September 2016.