Processed and analyzed human biological samples, including blood, bronchoalveolar lavage (BAL), and sputum, ensuring accurate and reliable sample preparation
Performed cell culture experiments using primary human bronchial and nasal epithelial cells, including air-liquid interface (ALI) culture, to investigate donor-specific responses
Conducted flow cytometry (Fortessa III) involving cell staining, running, and data analysis to evaluate immune and cellular markers
Utilized molecular biology techniques such as qPCR and ELISA to assess gene expression and protein levels, contributing to the identification of differential responses among donors
Performed statistical analysis and data visualization to interpret experimental results and present findings in team meetings and reports
Graduate Research Assistant
Blizard Institute
05.2023 - 12.2023
Explored differences in immune cell population and senescence marker expression between younger and older asthma patients under the supervision of Dr Emma Chambers
Performed in vitro assays on freshly isolated and cryopreserved PBMC as well as serum samples using a variety of techniques, including cytometric-bead array flex, allergen stimulation assay with cell trace vial, proliferation assessment and human TSLP ELISA.
Performed whole blood staining, cell types were assessed using flow cytometry (Aurora, LSR II,Novocyte)
Immune POCT Research Group Intern
VivaChek Biotech
07.2021 - 08.2021
Assisted in accuracy/specificity testing and quality control measures of SARS-CoV-2 rapid tests, including VivaDiag SARS-CoV-2 Neutralizing Antibody FIA Test Kit etc
Carried out gold immunochromatography assay and ELISA
Completed trainings on PCR, immunochromatography detectors and film-drawing and spraying instrument
Undergraduate Research Assistant
King's College London
09.2021 - 01.2022
A study analyzing the correlation between characteristics of Ara h 2-specific IgE and mast cell activation in a cohort of children with peanut allergies and peanut sensitization under the supervision of Professor Alexandra Santos.
Performed ImmunoCAP tests with patient serum and mast cell activation tests using flow cytometry.
Education
MSc - Translational and Experimental Immunology
University College London
London
09.2022 - 09.2023
BSc - Biomedical Science
King’s College London
London
09.2019 - 06.2022
Skills
Agarose embedding
Data analysis and softwares
Flow cytometry (Aurora, Fortessa III, LSR II,Novocyte)
Human sample processing (blood, BAL, sputum etc)
In vitro assays (CBA flex, ELISA, qPCR etc)
Primary cell culture
Publications
Santos A, Huang Y, et al. Ara h 2-specific IgE predicts mast cell activation in children. J Allergy Clin Immunol Pract. 2023. DOI.
Huang Y, et al. Senescent immune cells in older severe asthma patients impair immunity. BMJ Journals. 2024. DOI.