Overview
Work History
Education
Skills
Publications
Timeline
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Yue Huang

London

Overview

4
4
years of professional experience
4
4
years of post-secondary education

Work History

Research Technician

Imperial College London
02.2024 - Current
  • Processed and analyzed human biological samples, including blood, bronchoalveolar lavage (BAL), and sputum, ensuring accurate and reliable sample preparation
  • Performed cell culture experiments using primary human bronchial and nasal epithelial cells, including air-liquid interface (ALI) culture, to investigate donor-specific responses
  • Conducted flow cytometry (Fortessa III) involving cell staining, running, and data analysis to evaluate immune and cellular markers
  • Utilized molecular biology techniques such as qPCR and ELISA to assess gene expression and protein levels, contributing to the identification of differential responses among donors
  • Performed statistical analysis and data visualization to interpret experimental results and present findings in team meetings and reports

Graduate Research Assistant

Blizard Institute
05.2023 - 12.2023
  • Explored differences in immune cell population and senescence marker expression between younger and older asthma patients under the supervision of Dr Emma Chambers
  • Performed in vitro assays on freshly isolated and cryopreserved PBMC as well as serum samples using a variety of techniques, including cytometric-bead array flex, allergen stimulation assay with cell trace vial, proliferation assessment and human TSLP ELISA.
  • Performed whole blood staining, cell types were assessed using flow cytometry (Aurora, LSR II,Novocyte)

Immune POCT Research Group Intern

VivaChek Biotech
07.2021 - 08.2021
  • Assisted in accuracy/specificity testing and quality control measures of SARS-CoV-2 rapid tests, including VivaDiag SARS-CoV-2 Neutralizing Antibody FIA Test Kit etc
  • Carried out gold immunochromatography assay and ELISA
  • Completed trainings on PCR, immunochromatography detectors and film-drawing and spraying instrument

Undergraduate Research Assistant

King's College London
09.2021 - 01.2022
  • A study analyzing the correlation between characteristics of Ara h 2-specific IgE and mast cell activation in a cohort of children with peanut allergies and peanut sensitization under the supervision of Professor Alexandra Santos.
  • Performed ImmunoCAP tests with patient serum and mast cell activation tests using flow cytometry.

Education

MSc - Translational and Experimental Immunology

University College London
London
09.2022 - 09.2023

BSc - Biomedical Science

King’s College London
London
09.2019 - 06.2022

Skills

Agarose embedding

Data analysis and softwares

Flow cytometry (Aurora, Fortessa III, LSR II,Novocyte)

Human sample processing (blood, BAL, sputum etc)

In vitro assays (CBA flex, ELISA, qPCR etc)

Primary cell culture

Publications

  • Santos A, Huang Y, et al. Ara h 2-specific IgE predicts mast cell activation in children. J Allergy Clin Immunol Pract. 2023. DOI.
  • Huang Y, et al. Senescent immune cells in older severe asthma patients impair immunity. BMJ Journals. 2024. DOI.

Timeline

Research Technician

Imperial College London
02.2024 - Current

Graduate Research Assistant

Blizard Institute
05.2023 - 12.2023

MSc - Translational and Experimental Immunology

University College London
09.2022 - 09.2023

Undergraduate Research Assistant

King's College London
09.2021 - 01.2022

Immune POCT Research Group Intern

VivaChek Biotech
07.2021 - 08.2021

BSc - Biomedical Science

King’s College London
09.2019 - 06.2022
Yue Huang