Liam Brown

Accomplished and Efficient former University student with a strong understanding of most Diagnostic and analytical techniques for both disease identification and research. With years of university laboratory experience, I have honed my skills as a prospective biomedical scientist and now I am proficient with the following techniques.

PCR, Electrophoresis, DNA Transfection, Haematology, Plasmid Design, Primer Design, Western Blotting, Enzyme activity analysis, Cell culturing, Bioinformatics, Flow Cytometry, Immunohistochemistry, Aseptic Technique, DNA microarrays, ELISA

Offering background in scientific study and eagerness to learn and develop within laboratory setting. Brings aptitude for understanding complex scientific concepts and applying laboratory techniques with precision. Ready to use and develop skills in data analysis and laboratory procedures in Medical Laboratory Assistant role.

• Antibody Cloning
• Tissue Staining
• Mouse conditional knockout
• Gram staining
• Analytical Profile Index
• Tissue Storage

During my undergraduate, I assisted Dr. Vassilios Beglopolous and his PhD students who were conducting research on the potential epigenetic origin of Alzheimer's disease. This research was conducted with a mouse model, where two mice populations were examined using histological, proteomic and immunohistochemical techniques., The inspiration for the experiment was based on a paper by Tao Lu et al in 2014. This paper discovered a statistically significant relationship between Neurodegeneration and the pre-natal conditional knockout of the gene Neuro-Restrictive silencing factor., The experiment I assisted in was analysing qualitatively and quantitatively the absence of neurons when viewed under a microscope. A western blot was also conducted to see if proteins associated with neuronal and synaptic function were absent. This combined methodology allowed for a deep investigation into the molecular underpinnings of the disease. Histological analysis provided invaluable insights into the pathological changes in brain tissue, while Western blot experiments enabled quantification of specific protein markers associated with Alzheimer's pathology. The data was then collected and analysed using SPSS.