Jean Panackal
Cell Culture Scientist
Stevenage,Hertfordshire
Summary
Motivated and technically adept Cell Culture Scientist with extensive hands-on experience in iPSC-derived cell midels, primary cell lines and advanced co-culture systems. Currently employed at PerkinElmer on assignment at GSK, where I work collaboratively with GSK scientists and contribute to the development of physiologically relevant disease models to support target identification, validation and early stage drug discovery.
Overview
3
3
years of professional experience
4
4
years of post-secondary education
Work history
Cell Culture Scientist
PerkinElmer, on assignment at GSK
Stevenage, Hertfordshire
11.2023 - Current
- Hands-on experience in the maintenance and differentiation of induced pluripotent stem cells (iPSCs), primary cell lines and advanced direct and indirect co-culture systems.
- Currently contributing to the development of disease-relevant in vitro models for target identification and validation in translational research.
- Proficient in a wide range of laboratory and molecular biology techniques such as:
- Flow cytometry, qPCR, RT-PCR and western blotting.
- Immunofluorescence staining, high content imaging and microscopy using the Opera Phenix and Incucyte.
- Whole cell and cell-surface proteomics assays.
- Single-cell RNA Sequencing (scRNA-seq) experiments to analyse gene expression at single-cell resolution.
- Cell viability and proliferation assays and differentiation protocols.
- Automated workflows using robotic liquid handling systems such as Dragonfly, BRAVO, ViaFlo and Firefly for high-throughput experiments.
- Experience in writing and following Standard Operating Procedures (SOPs) and operatin under GLP and GDPR compliance.
- Strong background in scientific communication, with experience in:
- Designing and presenting posters and experimental data at scientific conferences.
- Producing detailed technical reports and maintaining research documentation.
Summer Research Intern
University of Hertfordshire
Hatfield, Hertfordshire
04.2023 - 07.2023
- Collaborated with Master's and PhD students on ongoing cell-culture based projects.
- Practiced aseptic techniques and maintained mammalian cell cultures.
- Performed cell culture hood santitation and adhered to biosafety standards.
- Performed media changes and subcultured suspension cultures.
- Exected western blotting and agarose gel electrophoresis for protein and DNA analysis.
- Applied chromatographic techniques for sample separation and analysis.
Research Assistant
University College London (UCL)
London, City of London
05.2022 - 09.2022
- Conducted experiments to study the effects of fluphenazine on the dopaminergic signalling pathway in C.elegans egg-laying behaviour.
- Adhered to Good Laboratory Practice (GLP) and quality control standards.
- Performed risk assessments and followed Standard Operating Porcedures.
- Participated in hazard identification and mitigation through generating COSHH forms.
- Applied single-cell RNA sequencing techniques to analyse gene expression.
- Compiled a comprehensive research report and presented experimental findings at conferences and departmental meetings.
Education
Bachelor of Science - Molecular Biologu
University of Hertfordshire
Hatfield 09.2019 - 09.2023
Skills
- iPSC Culture and Differentiation
- Primary Cell Line Maintenance
- Adherent and Suspension Cell Culture
- Co-culture System Development
- Aseptic technique
- High-Content Imaging
- Immunofluorescence
- Flow Cytometry
- Quantitative and RT-PCR
- Standard Operating Procedures (SOPs)
- Good Laboratory Practice (GLP)
- Laboratory Automation
- Inventory and Equipment Management
- Scientific Report Writing
- Cross-Functional Team Collaboration
Timeline
Cell Culture Scientist
PerkinElmer, on assignment at GSK
11.2023 - Current
Summer Research Intern
University of Hertfordshire
04.2023 - 07.2023
Research Assistant
University College London (UCL)
05.2022 - 09.2022
Bachelor of Science - Molecular Biologu
University of Hertfordshire
09.2019 - 09.2023
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