Amanda ElSamahy
Middlesbrough,North Yorkshire
Summary
A detail-oriented professional with a strong foundation in analytical skills and technical proficiency, complemented by exceptional communication and problem-solving abilities. Demonstrates expertise in safety awareness and inventory management, ensuring efficient operations and resourcefulness in dynamic environments. Adept at team collaboration and adaptability, with a focus on meticulous documentation and time management to drive organisational success.
Overview
10
10
years of professional experience
13
13
years of post-secondary education
Work history
Cell Culture Scientist
Histocyte Laboratories
Newcastle
2024.06 - 2026.04
- Assisted in simultaneous cultivation of various cancer cell lines.
- Conducted quality control testing using immunohistochemistry and microtomy.
- Helped with writing and updating SOPs and WIs.
- Completed assigned tasks to meet objectives accurately and efficiently.
- Aided in management of consumables using Kanban system.
- Manufacturing final products, by harvesting, embedding and sectioning.
- Managing freeze down stocks and LN2 refills.
- Handled additional responsibilities while effectively designating tasks, managing time and supporting team.
- Facilitated handling of CAPAs, NCs, and R&D tech transfers.
- Implemented process improvements to enhance efficiency.
Marine Fuel Technician
Lloyds Register
Redcar
2023.09 - 2024.05
- Ensuring quality and compliance of marine fuels used in shipping operations.
- Conducting thorough analysis and testing of fuel samples in fast paced environment
- Interpreting results and provided reports in timely manner.
- Guaranteed that vessels receive fuel of highest quality, meeting industry standards and environmental regulations.
- Maintained daily maintenance logs, updating data with accuracy to inform management of operational needs.
Assay Technician
QuantuMDx
Newcastle
2022.05 - 2023.08
- Assisted in development of IVD products for diagnostic platform Q-POC.
- Contributed to development of point of care assays for tuberculosis, SARS-CoV-2, and STIs in fast-paced environment with diverse sample types.
- Participated in planning experiments and creating reagents, including buffers, through filtration equipment and nucleic acid extraction.
- Supported data analysis, report writing, and collaborative efforts with assay team and engineers.
- Ensured maintenance of stocks and cleanliness within laboratories.
- Presented data to line managers and stakeholders.
- Followed protocols for handling and disposal of human samples according to HTA guidelines.
Postgraduate Research
The DMD Neuropsychiatric Syndrome
Northampton
2017.07 - 2021.07
- Supported research into Duchenne brain by collaborating with additional patients.
- Assisted in bioinformatics analysis for associations with DMD gene (Dp71 isoforms) and its impact on cognitive impairment in patients.
- Designed primers for PCR experiments.
- Documented lab work meticulously and presented findings to supervisors and postgraduate department regularly.
- Secured funding for research through preparation of scientific bids and project proposals.
- Complied with RAs SOPs and adhered to project timelines.
- Maintained laboratory stock and equipment, including incubators and fume hoods.
- Addressed infection issues and resolved failed assays effectively.
- Coordinated collaborations with Dubowitz Biobank, UCL and Life Centre, Newcastle.
Undergraduate Research
Duchenne Muscular Dystrophy
Northampton
2016.10 - 2017.04
- Modelled loss of dystrophin isoform (Dp71) and measured levels from DMD patient fibroblasts alongside their cognitive and behavioural symptoms
- Assisted in siRNA-mediated knockdown in neuronal and glial cell lines to analyse gene mutations..
- Conducted mammalian tissue culture, thawing and freezing cells, passaging cells
- Using RNA extraction, RT-PCR and scratch assays exhibiting differences in cell migration and proliferation, in association with cognition
- Analysis using ImageJ, SPSS statistics and interpretation of results
- Problem solving failed assays and finding improvements.
Intern
John Walton Muscular Dystrophy Research Centre
Newcastle
2016.05 - 2016.09
- Explored dystrophin in CNS and skeletal muscle
- Executed end-to-end protein characterization workflows, including BCA quantification, SDS-PAGE, and Western Blotting on skeletal muscle lysates.
- Demonstrated proficiency in membrane processing (PVDF/Nitrocellulose), utilizing Chemiluminescence (X-ray) detection and specialized stripping/re-probing protocols with Ponceau S and Coomassie staining.
- Managed HEK cell lines (passaging, overexpression) and performed high-resolution Immunocytochemistry (ICC) using Phalloidin, DAPI, and targeted antibodies for Confocal and Axio Imager microscopy.
- Investigated protein expression (IQGAP1, Cav3, SIL1) in mouse models for Limb-Girdle Muscular Dystrophy (LGMD) and GFPT1 mutations to identify biomarkers of muscle pathology.
Education
MPhil - Molecular Biology
The University of Northampton
Northampton 2017.10 - 2021.07
BSc (Hons) - Human Bioscience
The University of Northampton
Northampton 2014.10 - 2017.07
A level -
Newcastle College
Newcastle 2012.09 - 2014.06
GCSE -
Emmanuel College
Gateshead 2007.09 - 2012.06
Skills
- Analytical Skills
- Attention to Detail
- Technical Proficiency
- Safety Awareness
- Communication Skills
- Problem-Solving
- Time Management
- Team Collaboration
- Adaptability
- Documentation Skills
- Resourcefulness
- Inventory management
Websites
Accomplishments
- 04/2018, Presented to UK Neuromuscular Translational Research Conference, Cambridge
- 03/2018, Postgraduate Research Award 2018, University of Northampton
- 03/2018, Abstract published by UK Neuromuscular Translational Research Conference, Cambridge
- 09/2017, Secured funding with NCOP, hefce and crowdfunding on Experiment.com
- 04/2017, Dissertation published as an abstract to the World Muscle Society 2017, Saint Malo
- 04/2017, Human Bioscience Dissertation Poster Presentation Winner
Volunteering
01/2017 - 04/2020, STEM Ambassador/STEAM Changemaker - Engaging with children and young people about science and biochemistry to encourage them in their future careers.
05/2017 - 05/2018, Volunteering & Social Enterprise Committee Chair - Problem solved, negotiated, shared ideas, represented the committee and held people accountable at Student Council.
05/2017 - 05/2018, President, Marrow (Northampton) - Managed activity and ensured we conform to SU and Anthony Nolan practises, encouraged others to get involved and register.
11/2016 - 05/2017, Donor Recruitment Coordinator, Northampton Marrow - Organised and assisted Anthony Nolan events, including fundraising and handling swab and sputum samples.
10/2016 - 07/2017, Course Representative - Represented students and negotiated with staff to problem solve, as well as planning, minuting and chairing SSLC meetings.
Timeline
Cell Culture Scientist
Histocyte Laboratories
2024.06 - 2026.04
Marine Fuel Technician
Lloyds Register
2023.09 - 2024.05
Assay Technician
QuantuMDx
2022.05 - 2023.08
MPhil - Molecular Biology
The University of Northampton
2017.10 - 2021.07
Postgraduate Research
The DMD Neuropsychiatric Syndrome
2017.07 - 2021.07
Undergraduate Research
Duchenne Muscular Dystrophy
2016.10 - 2017.04
Intern
John Walton Muscular Dystrophy Research Centre
2016.05 - 2016.09
BSc (Hons) - Human Bioscience
The University of Northampton
2014.10 - 2017.07
A level -
Newcastle College
2012.09 - 2014.06
GCSE -
Emmanuel College
2007.09 - 2012.06