TEJASWINI KANCHANAPALLY

PROJECT/RESEARCH ASSISTANT

Council of Scientific, CSIR-IICT

Hyderabad, India

04.2015 - 03.2017

Conducted mammalian cell culture experiments, maintaining multiple cell lines, optimizing growth conditions, and ensuring the viability of cells for various assays.
Proficiently executed cytotoxicity screening assays using a variety of techniques, including SRB, MTT and LDH assays, to assess the effects of compounds on cell viability.
Screened more than 3000 compounds from CSIR-IICT drug compound library.
Demonstrated strong animal handling skills, responsible for the care and maintenance of laboratory animals, including dosing through various routes (oral, intravenous, intraperitoneal, etc.) and monitoring their health.
Executed DNA extractions, PCR amplifications, DNA sequencing, gene expression, recombinant DNA technology and gel electrophoresis, contributing to genetic research projects.
Conducted Western blotting experiments, performing protein extraction, electrophoresis, and blotting techniques to analyze protein expression and signaling pathways.
Proficient in molecular biology techniques such as cloning, plasmid preparation, and transformation and stable cell line generation.
Assisted with histopathological studies, preparing tissue samples, performing staining procedures, and analyzing microscopic images.
Conducted biochemical analyses, including ELISA and enzymatic assays, to quantify specific biomarkers and assess biological processes.
Designed and executed IN-VIVO toxicity studies, including acute toxicity assessment, following regulatory guidelines and ethical considerations.
Evaluated blood and serum biochemical parameters and histopathological observation of tissue sections.
Utilized data analysis software to process and interpret experimental results, generating meaningful insights from complex datasets using GraphPad Prism, Microsoft Excel.
Maintained meticulous documentation of experimental procedures, results, and laboratory protocols, ensuring compliance with research standards and regulations.