Sriswetha GS

• Performed fed-batch mammalian cell culture using Wave, STR, and SUB systems.
• Led and supported process characterisation studies for BLA programs.
• Conducted revival, cryopreservation, and stability studies following SOPs and GMP standards.
• Prepared platform and custom media/feeds; adapted cell lines to defined media.
• Authored protocols and technical reports for regulatory submissions.
• Operated cGMP analytical equipment including NOVA, Micro Osmometer, Vi-Cell BLU/XR.
• Wrote risk assessments and COSHH assessments for media/raw materials.
• Managed data according to ALCOA+ principles and maintained GMP documentation.
• Coordinated equipment servicing, calibration, and parts replacement.
• Updated software templates for IMS, iLab, and Item Tracker.
• Forecasted reagents, managed consumables, and coordinated procurement.
• Collaborated with downstream and analytical development teams.
• Trained new staff and provided support for weekend critical experiments.

• Investigated human embryonic stem cell antibody labeling of glioblastoma stem cells.
• Performed aseptic culture of cancer cell lines and robotic liquid handling operations.
• Analyzed high content imaging data using MetaXpress and generated reports using GraphPad Prism.
• Maintained structured experiment documentation including graphs and flowcharts.

• Knocked out hbbe:1 gene to model human anemic disease in zebrafish using CRISPR/Cas9 technology
• Identified zebrafish version of gene using ENMSEMBL and CHOPCHOP to identify the target site
• Isolated, purified the DNA by performing PCR and Agarose Gel electrophoresis (AGE) to analyze the changes in hbbe:1 gene in the zebrafish embryo using the microscope

• Extracted Bacterial DNA and performed PCR and Agarose Gel electrophoresis (AGE) for the bacteria E.coli
• Performed Antibiotic sensitivity test with different antibiotic discs tested for E.coli and Bacillus subtilis
• Gained experience on ELISA to identify antibodies in E.coli and detected antibodies using spectrophotometer
• Theoretical knowledge on Flow cytometry, SDS-PAGE and Western Blotting

• Prepared microbiological media and solutions for laboratory experiments.

• Extracted bacterial DNA; performed PCR and AGE.
• Conducted antibiotic sensitivity assays for E. coli and B. subtilis.
• Performed ELISA and spectrophotometric analysis.
• Learned theoretical concepts of flow cytometry, SDS-PAGE, and Western blotting.