Sriparna Chakraborty

School of Molecular Bioscience (College of Medical, Veterinary and Life Sciences)

• Project: Comparative Analysis on Expression Pattern of Parkin Splice variant M4T4F2
• Supervised by: Dr. Helen Walden, Dr. Joanna Koszlea
• Domain of Study: Recombinant DNA technology, Proteomics, Bioinformatics, Computation
• The project aims to characterize selective splice variants of Parkin.
• Recombinant DNA technology was used to express the wild-type protein and the isoforms. Purification of protein was performed using HPLC (Three steps of purification are followed: Affinity chromatography, Size Exclusion, and Ion Exchange chromatography)
• Accomplishment: Successfully expressed and purified wild-type Parkin and the M4T4F2 splice variant. Protein expression was confirmed by Western blotting and SDS-PAGE analysis. A ubiquitination assay validated the activation stages of canonical wild-type Parkin. Both experimental and computational analyses indicated significant insolubility of the M4T4F2 protein.

Experiment: Identifying Neuroglian Gene Mutation in icebox phenotype Drosophila melanogaster.

• Domain of study: DNA extraction and purification, recombinant DNA technology, DNA sequencing, and bioinformatics analysis
• Accomplishment: The experiment affirmed the successful recombination of the gene of interest and justified the Neuroglian gene mutation in icebox phenotype Drosophila melanogaster.

Experiment: The Effect of Doxorubicin on Expression of P53 and P21 in Breast Cancer Cells

• Domain of study: Gene expression analysis (Operational experience on RNA isolation, cDNA synthesis techniques, Reverse Transcription and RT- qPCR), Proteomics(Quantitative and qualitative estimation of protein, SDS-PAGE and Western blotting)
• Accomplishment: The study established that in Doxorubicin-treated cells, p21 and p53 expression levels are not correlated. Therefore, we propose further validation of Ras expression as a potential regulator of the p53–p21 pathway.

• Internship domain- Molecular Biology (Tools of molecular biology)
• Experienced in genomics studies (both wet and dry lab), including DNA extraction and purification from bacterial and animal cells, PCR and primer design using bioinformatics tools, Agarose gel electrophoresis, DNA cleanup, and recombinant DNA techniques such as PCR product cloning, bacterial transformation, and analysis of transformed cells.
• Proteomics technology (protein extraction and purification), focusing on quantitative (Bradford assay: colorimetric estimation of a desired protein) using a UV-spectrophotometer, computational analysis of protein structure, protein-protein interactions, and drug-protein interaction
• Industrial microbiological techniques, basics of microbiology (bacterial growth optimization and analysis), DNA isolation technique, Transformation of bacterial cells in terms of recombinant DNA technology study, Antimicrobial resistance in the context of drug development
• Study covered as an outcome of research project: The effect of E. coli-generated Silver nanoparticles on the DNA of mutative Staphylococcus aureus (mutative strain MRSA ST22)

Accomplishment

• Presented findings from data analysis tasks clearly to non-technical colleagues.
• Improved team communication by organising regular team meetings and discussions.
• Increased team productivity by taking diligent meeting minutes.

• Department of Neurophysiology
• Systematic Review: NLRP3 Inflammasome-mediated stress response to develop Neuropsychiatric disorders