Soroush Shahgholi

• Treated wild-type and Mutant chronic myeloid leukaemia cell lines with paroxetine and its combination with imatinib
• Investigated cell cycle arrest and apoptosis of treated cells using FACS
• Examined impact of this combination on protein expression levels of significant survival pathways such as MEK/ERK using western blotting
• Performed data analysis tasks, providing clear and concise findings.
• Adhered strictly to lab safety protocols, maintaining a secure work environment.
• Maintained meticulous records of all experiments conducted, bolstering reliability of findings.

I worked as an assistant at Dr. Saghaei’s laboratory at Shahrekord University of Medical Sciences, Aiding medical students with 2 main thesis projects:

• Effect of Cuminaldehyde on Depression-Like Behaviours Induced by Chronic Unpredictable Stress: Role of CRF and Its Receptors
• Urolithin B and depression-like behaviour after adolescent social isolation in mice: BDNF/mTOR pathway involvement
• Executed molecular and cell biology experiments: cell culture, western blotting, and RT-PCR
• Optimizing protocols in collaboration with senior fellows, resulting in enhanced speed and accuracy of experiment execution.
• Increased safety standards with regular risk assessments.

• Conducted daily cell culture tasks using L929 and MCF7 cell lines.
• Performed MTT assays to assess cell viability.
• Maintained laboratory safety protocols and cleanliness standards.
• Supervised junior interns, ensuring adherence to lab practices.
• Adapted to new situations and learned new skills quickly.
• Participated in training and seminars to enhance skills and promote growth.
• Showed initiative and willingness to take on new challenges.