Siddhant Dhende

Dedicated and detail-oriented Research Assistant with expertise in molecular and cellular research methodologies, data analysis, and project coordination. Skilled in literature reviews, executing experiments, and laboratory procedures. Proficient in inducing protein mutations and performing comprehensive research projects. A proactive team player with strong organizational skills, committed to advancing scientific inquiry and expanding knowledge in the field.

• Proficient in genetic and molecular biology research methodologies, including qualitative and quantitative approaches, and data analysis with statistical interpretation
• Exceptional organizational abilities for multitasking, meeting project deadlines, managing experimental protocols, and troubleshooting
• Effective communication skills for team collaboration, presenting findings, and writing detailed reports
• Detail-oriented in literature reviews, synthesizing information, identifying research gaps, and adhering to research ethics and regulatory standards
• Skilled in bioinformatics tools for enhanced data collection, sequence analysis, and structural prediction
• Lab management including ordering supplies, maintaining stock levels, and ensuring equipment safety

• Master’s Project, Performed research project at the University of Sussex, inducing mutations in the hinge region of Smc6 protein in fission yeast (Schizosaccharomyces pombe). Conducted numerous DNA purifications, PCR reactions, and Gibson Assembly procedures. Investigated the impact of mutations on DNA repair and stress response, resulting in a better understanding of SMC5/6 proteins. Explored roles of SMC5/6 in genetic recombination and dynamic regulation in DNA damage response, presenting findings at the university's Academic presentation assessment. Executed a group research project involving DNA cloning, site-directed mutagenesis, PCR, bacterial transformation, and protein analysis techniques. Demonstrated proficiency in bioinformatics tools for sequence analysis and structure prediction.
• Bachelor's Project, Performed research project on milk adulteration, identifying adulteration in 100% of analysed milk samples. Conducted over 10 biochemical tests, including alcohol precipitation, boric acid, formaldehyde, and starch detection. Presented project findings at a local food safety seminar, raising awareness about milk adulteration issues.
• Student Research intern at Lac Operon Pvt.Ltd – Pune, Performed a developmental biology project investigating chick embryo developmental stages, analyzing embryonic samples. Assisted in designing and executing experiments, discovering morphological features of chick embryos. Presented research findings at three college seminars, sparking interest and engagement among students and faculty.