Shalu Joseph

I possess two years of industrial experience in cloning, yeast strain engineering, protein expression, functional analysis, lab management, and executing routine and complex experiments. I exercise great attention to detail, ensuring accuracy and consistency in my personal performance, collaborative teamwork and on-time completion of projects. Moreover, I offer analytical thinking, creative problem-solving, the ability to learn and grow and scheduling flexibility.

I have worked on a project, ‘Application of aromatically tuned chimeric sensor protein of the two-component system in novel antibiotic screening' under Dr Roger Draheim's supervision at the University of Portsmouth , employing synthetic microbiology, biochemistry and biophysics to create a chimeric sensor protein in a fluorescence reporter system. I use aromatic tuning to modulate signal output from two-component signalling circuits in a stimulus-independent manner. I am trained in methods like primer designing, site-directed mutagenesis by SDM-PCR, bacterial DNA sequencing analysis using snap gene software, DNA quantification using Nano-Drop spectrophotometer, different recombinant DNA techniques like plasmid isolation by mini-prep, restriction digestion, ligation, and transformation, gel electrophoresis, protein expression analysis by western blot, quantification using Image J and QtiPlot, and signal output analysis by fluorescent Spectrophotometry.

I have worked with Dr Murphy Wan on a paper “Schisandrin B Suppresses Colon Cancer Growth by Inducing Cell Cycle Arrest and Apoptosis: Molecular Mechanism and Therapeutic Potential.” As part of this study, I have trained in human cell culture and protein assays.

I had worked on a project, ‘Analysis of SUMOylation of Smc1in a SUMOylation defective mutant strain of Saccharomyces cerevisiae ' , which required learning basic techniques in yeast molecular genetics and biochemistry at Professor Shikha Laloraya's lab at the Indian Institute of Science using a mutant yeast strain, expected to be defective in SUMOylation, a post-translational protein modification. I demonstrated deficient SUMOylation of a chromosomal structural maintenance protein by affinity chromatography (Ni-NTA pull-down) and western blotting. This study is part of a paper that is in the publishing pipeline, titled "Cohesin sumoylation is required for repression of subtelomeric gene expression in Saccharomyces cerevisiae" (10.1101/2025.05.26.656218).