Sakar Hosseini

Skilled Research and Laboratory Technician specializing in molecular biology, including cell culture, qPCR, ELISA, Western blotting, and flow cytometry. Demonstrates strong data analysis capabilities and proficiency in Microsoft Office for clear result presentation. Known for exceptional attention to detail, collaborative teamwork, and efficient time management. Reliable, adaptable, and comfortable working in fast-paced lab settings.

• Mastering Laboratory Skills – Imperial College London, Faculty of Medicine
• Good Clinical Practice (GCP) – NIHR
• Laboratory Management Systems Essentials – Udemy
• NGS Data Analysis and Whole Genome Sequencing (WGS) - Nexintek, Canada
• Molecular Cloning: From Design to Implementation – Nexintek, Canada

Brewing Fermentations using Active Dried Yeast: Analysing Fermentation Performance and Flavour Stability – University of Nottingham

• Analysed fermentation performance, yeast viability, and flavour stability in 5 Saccharomyces cerevisiae strains using serial repitching (G0–G3).
• Found ADY showed up to 30% improved fermentation efficiency, with viability increasing from ~75% to >94%after initial adaptation.
• Used methylene blue staining, CO₂ loss tracking, and APCI-MS to measure kinetics and flavour consistency.
• Results supported industrial reuse of ADYin brewing, with stable performance from G1 onwards

 Expression of key genes in monoterpene biosynthesis in Lavandula angustifolia under abiotic stress and hormonal treatment – University of Guilan (Published in the National Journal of Biology – 2 papers)

• Studied gene expression in lavender (Lavandula angustifolia) under UV stress, drought, and treatment with salicylic acid (SA) and methyl jasmonate (MeJA).
• Performed RNA extraction, cDNA synthesis, and semi-quantitative RT-PCRtargeting borneol, fenchol, and trans-α-bergamotene synthase genes.
• Found MeJA upregulated gene expression up to 3-fold; UV also enhanced gene activity, while drought suppressed expression.
• Results showed potential for boosting essential oil production through targeted elicitor treatments.

• Graduate Route Visa valid until 31 December 2026
• Available for full-time roles across the UK; willing to relocate

References available upon request