Rohit Thakur
Nottingham
Summary
Results-driven biotechnology professional with three years of specialized experience in HEK293 cell culture, plasmid DNA production, and recombinant antibody purification. Proficient in advanced chromatography techniques, including Protein A, IEX, and SEC, as well as TFF and AKTA systems within GMP-compliant manufacturing settings. Expertise in quality control analysis, BMR documentation, SOP adherence, and process optimization ensures high-yield and reproducible results. Proven ability to maintain attention to detail while demonstrating adaptability and strong teamwork skills in cross-functional scientific environments enhances collaborative project success.
Overview
3
3
years of professional experience
1
1
Certification
Work History
Executive, Gene Therapy
Reliance Life Sciences
India
11.2021 - 12.2023
- Delivered robust HEK293 cell culture performance through meticulous resuscitation, subculture, cryopreservation, and transfection, demonstrating strong attention to detail and process discipline.
- Executed plasmid DNA purification using alkaline lysis, chromatography (affinity, ion exchange, gel filtration) on AKTA system, Depth Filtration and TFF (UF/DF).
- Prepared sterile growth media, buffers, and reagents under strict aseptic conditions, ensuring compliance with SOPs, GMP principles, and ISO standards.
- Maintained 100% accuracy in Batch Manufacturing Records (BMRs) and electronic laboratory notebooks, strictly adhering to SOPs and GMP standards to ensure full traceability.
- Executed process optimisation and continuous improvement activities, improving yield and reproducibility.
- Conducted QC analyses using gel electrophoresis, HPLC and Nanodrop/UV-Vis spectrophotometry.
- Supported continuous improvement initiatives through accurate data collection, trend analysis, and clear communication of results to scientists and production teams.
- Led cross-functional teams to enhance operational efficiency and streamline project delivery.
Trainee Executive, DSP
Intas Biopharmaceutical
India
09.2019 - 11.2020
- Managed large-scale purification workflows for recombinant antibodies, employing affinity chromatography techniques to achieve optimal purity in biological solutions.
- Conducted quality control tests to ensure compliance with industry standards.
- Collaborated with cross-functional teams to streamline manufacturing processes.
- Oversaw operation and maintenance of Akta chromatography systems, focusing on ion exchange and gel filtration to guarantee reliable purification processes and system integrity.
- Performed Tangential Flow Filtration (TFF) and depth filtration for the concentration and diafiltration of biologics, maintaining product integrity throughout the process.
- Prepared complex buffers and growth media supplements in strict adherence to SOPs, ensuring chemical accuracy and sterile conditions for production.
- Executed meticulous documentation of all manufacturing activities in batch manufacturing records (BMRs) to support adherence to rigorous CGMP and ISO standards.
- Collaborated with upstream teams to facilitate seamless process handovers, identifying opportunities to streamline downstream processing and improve overall manufacturing yield.
Education
Master of Science (Distinction) - Biotechnology
Nottingham Trent University
Nottingham, United Kingdom01.2025
Bachelor of Engineering (Distinction) - Biotechnology
North Maharashtra University
India06.2019
Skills
- HEK293 cell line upkeep
- Cell Revival
- Passaging
- Resuscitation
- Subculturing
- Transfection
- Cryopreservation
- Viability Assessment
- Lysis
- Clarification
- AKTA Chromatography
- Affinity
- IEX
- SEC
- GFC
- TFF
- UF/DF
- Formulation & Storage
- SAP
- Minitab
- ELN
- Microsoft Office
- HPLC
- UV-Vis Spectroscopy
- Light Microscopy
- Gel Electrophoresis
- SDS-PAGE
- HSE
- GDP
- GLP
- SOP
- GMP
- BMRs
- Presentation Skills
- Communication Skills
- Proactive
- Teamwork
- Time Management
- Project Management
- Attention to detail
- Accountability
- Reliability
- Problem-solving
Certification
- Food Technology and Industrial Microbiology, Central Dogma, 2018
- Fermentation Technology, Mitcon Biopharma, 2017
Additional Information
Right to work in the UK., Willing to relocate and work on-site.
References
References are available upon request.
Timeline
Executive, Gene Therapy
Reliance Life Sciences
11.2021 - 12.2023
Trainee Executive, DSP
Intas Biopharmaceutical
09.2019 - 11.2020
Master of Science (Distinction) - Biotechnology
Nottingham Trent University
Bachelor of Engineering (Distinction) - Biotechnology
North Maharashtra University