Priyanka Ghongane

• Assisted clinical study teams with managing global study operations to support execution of studies on time and in compliance.
• Collaborated with study managers in facilitating start-up and conduct activities.
• Supported study planning and coordination.
• Helped develop and maintain study-level trackers, dashboards, timelines, and tools to ensure visibility.
• Coordinated logistics for investigator meetings, trial events, and site communications.
• Managed assigned start-up activities including system setup and document readiness to meet standards.
• Provided assistance for risk monitoring and issue management by raising early warning signals.
• Maintained and monitored clinical trial systems (eClinical) ensuring accurate data entry and reporting.
• Supported data collection oversight including query resolution and reconciliation activities.
• Managed trial documentation (e.g eTMF, eClinical) to ensure inspection readiness.

Management:

• Monitor project plans, timelines, and deliverables, manage documentation
• Managed project to implement electronic lab book for data synchronisation and inventory management using project management methodologies, processes, and best practice technology
• Managed project budgeting and timeline effectively
• Collaborated with colleagues and CROs to design and execute experiments
• Delivered training and mentorship to trainees and summer interns in gene therapy techniques

Technical:

• Developed PBMCs isolation protocols and optimised FACs based killing assays.
• Developed and implemented an 8-colour flow panel aligned with project objectives
• Conducted transfection and electroporation for multiple ongoing projects
• Compiled, interpreted and delivered experimental results to cross-functional teams within gene therapy projects
• Planned in-vivo studies and analysed data for proof of concept development

• Isolated, stimulated and expanded primary T cells as well as T-cell subtypes.
• Generated retroviral and/or lentiviral vectors and transduced target cells.
• Performed immune-based assays, including proliferation and cytotoxicity, to support CAR T-cell candidate selection
• Developed novel assays to investigate CAR T-cell function
• Specialised in panel design and optimisation for multicolour flow cytometry
• Optimised assay protocols alongside process development team
• Prepared CAR T-cell products for in vivo studies and analysed samples using Flow Cytometry

• Construction of customized plasmid/vector for cloning.
• Conducted bacterial transformation and maxiprep purification of plasmid DNA
• Prepared lentiviral and retroviral constructs for stable breast cancer cell lines in research projects
• Virus titration using FACS based GFP quantification.
• Executed transfection and transduction protocols for cell lines with siRNA and custom-developed viruses
• Conducted expression profiling of diverse cell lines using Western blotting techniques
• Purified and optimised in-house antibodies for targeted use

• Designed stable cell line model for APOBEC3B using doxycycline induction
• Performed high-throughput siRNA screen to analyse APOBEC3B's enzymatic activity
• Maintained cell lines through STR validation and mycoplasma testing from internal cell bank
• Delivered technical support for siRNA screens, Western Blotting, cloning and protein quantification tasks
• Presented research findings through oral and poster presentations at multiple events

• Conducted proteomic profiling of signalling pathways via western blotting
• Protein quantification by biochemical assays.
• Selecting homogenous population of cells by FACS analysis.

• Conducted overexpression and purification of protein complexes
• Constructed plasmids with tailored DNA inserts to facilitate study of protein complexes
• Purified plasmid DNA and transformed bacteria for subsequent research applications
• Executed PCR of target gene, resolving troubleshooting issues to ensure successful amplification
• Assisted in research projects at Oxford focused on biological and medical sciences