Years of research experience
Dr Pragati Pandey
Peterborough
Summary
Stem cell biologist and cardiovascular researcher with over 10 years of research experience across KCL, QMUL, UCL and Imperial. During those 10 years, I have led the establishment of two laboratories, supervised and trained over 20 researchers, and developed several novel assays leading to well-cited publications in high-impact factor journals. I have also presented at various national and international conferences and public outreach events. Through a passion for stem cell therapies, I have explored the efficacy and safety of stem cell-derived cells using several tissue engineering approaches in animal models including mice, rats, rabbits and pigs. With expertise in cellular and molecular biology, cardiovascular research and cell and gene therapy, I am looking for the right opportunity to utilise all my skills whilst progressing as an ever-growing scientist.
Overview
10
10
Work History
Senior Assay Scientist
California Institute Of Technology
Pasadena
01.2013 - 05.2016
- Trained and advised junior staff in the assay development group.
- Interacted with customers on assay projects and technology transfer.
- Worked closely with quality control for method development and authoring of SOPs for routine testing and lot release.
- Interacted with manufacturing group to troubleshoot assays and ease of transfer from Development to Production.
- Utilized new assays for research purposes to produce literature for marketing purposes.
- Worked with single-molecule detection platforms such as Singulex-Erenna and Quanterix Simoa-HD1.
- Developed cell-based qPCR assay for measuring bioactivity of cytokines in human serum.
Assay Scientist
Abbott Laboratories
Indianapolis
06.2011 - 12.2012
- Developed high-sensitivity immunoassays, such as ELISAs for commercial manufacture in to kits.
- Optimize and validate assay performance in multiple biological matricies from normal and diseased organisms.
- Performed routine mammalian cell-culture and cell cryopreservation to maintain cell lines for use in cell-based assays, flow cytometry, and monoclonal antibody screening.
- Purified monoclonal and polyclonal antibodies using affinity, ion-exchange, and size-exclusion chromatography on GE-Akta FPLC system.
- Characterization and QC of purified antibodies by UV-VIS spectroscopy, SDS-PAGE, western blotting, Iso-Electric Focusing, Isotyping etc.
- Determined potency/activity of antibodies and proteins using suitable cell-based assays.
- Phycoerythrin, biotin conjugation of antibodies for use in immunoassays and flow cytometry applications.
- Characterized antibody binding affinity and epitope binning using Octet-ForteBio for high-throughput binding pair selection.
Undergraduate Researcher
Rutgers Environmental Biophysics And Molecular Ecology
City
01.2009 - 01.2010
- Investigation of economically feasible biodiesel production by processing oil from Phaeodactylum tricornutum.
- Optimization of diatom lipid biosynthesis using bioinformatics and a systems biology approach.
- Gene expression profiling of cultured alga using real-time qPCR.
Extractions Technician
Accutest Laboratories
City
07.2007 - 11.2008
- Responsible for preparing sample extracts for GC/MS organic analysis of soil/water for organochlorine environmental pollutants.
- Preparation of sample extracts using continuous liquid/liquid extraction, ultra-sonication, pressurized fluid extraction, Separatory funnel, Soxhlet and other standard EPA test methods with cGLP.
Education
M.S - Chemical and Biochemical Engineering
Rutgers School of Environmental and Biological Sciences
New Brunswick, NJ2012
B.S - Biotechnology
2010
Skills
Laboratory: Cell culture, Molecular Cloning, Maintenance of mammalian cell lines, Flow cytometry (FACS), DNA/RNA extraction, Transfections, qPCR, Immunoassays such as ELISA, Octet ForteBio, Singulex-Erenna, Quanterix Simoa-HD1, Cell-based assays, Protein extraction and purification methods, SEC, IEX, FPLC and Affinity chromatography on GE/Pharmacia Akta system, SDS-PAGE, western blot, IEF, UV-VIS spectrophotometry, Microscopy *Computational: SoftMax Pro, GraphPad Prism, Beckman Coulter Kaluza, MATLAB, Python, Microsoft Office Suite
Publications
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Skills
approach, biology, cell-culture, Cell culture, DNA, ELISA, GC, IEF, Iso, marketing, MATLAB, exchange, Microsoft Office Suite, oil, Optimization, PAGE, Prism, Python, quality control, real-time, research, Preparation of sample, preparing sample, troubleshoot, UV, western blotting
Timeline
Senior Assay Scientist
California Institute Of Technology
01.2013 - 05.2016
Assay Scientist
Abbott Laboratories
06.2011 - 12.2012
Undergraduate Researcher
Rutgers Environmental Biophysics And Molecular Ecology
01.2009 - 01.2010
Extractions Technician
Accutest Laboratories
07.2007 - 11.2008
M.S - Chemical and Biochemical Engineering
Rutgers School of Environmental and Biological Sciences
B.S - Biotechnology
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