Developed leadership and team management skills in fast-paced, customer-oriented environment. Specialized in maintaining high service standards and optimizing operational efficiency. Seeking to transition into lab based work where these transferrable skills are useful in addition to my degree.
Time management
Inventory control
Resource allocation
Performance improvements
Ingredient preparation
Decision making
Team building
Quality control
Supply ordering
Team direction
Morale Building
Health and Safety compliance
Bacteria handling -
Wide range of media preparation; Solid and liquid cultures; Glycerol stock preparation; DNA and plasmid extraction, purification and quantification; Growth and viability assays; Standard and differential staining, imaging via microscopy; Transformation and selection of transformed microorganisms.
Yeast handling -
Wide range of media preparation; Solid and liquid cultures; Glycerol stock preparation; DNA extraction, purification and quantification; Growth and viability assays; Standard and differential staining, imaging via microscopy; Gene replacement via CRISPR; Preparation of chemically competent yeast; Transformation and selection of transformed strains.
Cell Culture -
Mammalian cell culture (HCT116), cell counting, viability, refreshing and proper disposal in line with the lab SOP.
Protein handling -
Western blot; Bradford assay; SDS-PAGE; Coomassie staining; ELISA
Molecular biology -
PCR, qPCR; Sanger Sequencing; Primer and plasmid design; Agarose gel electrophoresis, gel extraction and imaging; restriction digests and ligation.
Microscopy -
Sample and slide preparation; Light, phase, confocal and fluorescent microscopy and data analysis using ImageJ.
Miscellaneous -
SnapGene, ImageJ, BLAST, genome browsers/databases and MS office.