Nain Taara Bukhari

· Dedicated and detail-oriented professional with a strong passion for scientific inquiry and laboratory work, complemented by foundational knowledge in Clinical microbiology.

· Proven expertise as a Clinical Microbiologist, specializing in microbial identification, antimicrobial susceptibility testing, and laboratory diagnostics.

· Committed to fostering team collaboration and adapting to evolving challenges while ensuring accurate and timely diagnostic results.

Proficient in utilizing advanced laboratory techniques to enhance infection control measures and improve patient outcomes, with a solid understanding of regulatory standards and quality control practices that contribute to a safe and efficient laboratory environment

Laboratory and Diagnostic Skills  Specimen Handling & Processing  Infection Control & Bio-safety Advanced Techniques & Automation Data Interpretation & Quality Assurance Software & Reporting

• Culture Techniques: Aerobic and anaerobic bacterial cultures; fungal and mycobacterial cultures
• Microscopy: Gram staining, acid-fast staining, fungal morphology, parasitology slides
• Biochemical Identification: Manual (e.g., TSI, SIM) and automated systems (e.g., VITEK, BD Phoenix)
• Molecular Diagnostics: PCR, RT-PCR, GeneXpert, multiplex PCR platforms
• Antimicrobial Susceptibility Testing: Disk diffusion, MIC, E-test, automated systems (e.g., VITEK 2, MicroScan)
• Blood Culture Systems: BACTEC, BacT/ALERT interpretation and processing
• Proficient in processing specimens from blood, urine, CSF, respiratory, stool, wound, and sterile body fluids
• Chain-of-custody, sample storage, and transport protocols
• Infection prevention protocols (hospital-acquired infections surveillance, outbreak response)
• Biosafety Level 2 and 3 (BSL-2/3) laboratory practices
• Biorisk assessment and management
• Decontamination and waste disposal techniques
• ELISA, immunochromatographic tests, and rapid diagnostics
• Serological testing (Widal, Weil-Felix, ASO, HIV, HCV, HBV, Dengue)
• DNA/RNA Extraction and Qualitative and quantitative PCR performance
• Interpretation of lab results in clinical context
• Internal and external quality control (IQC/EQC)
• Laboratory Information Management System (LIMS) usage
• Root cause analysis and corrective action documentation
• Laboratory Information Systems (LIS) like SoftLab, Meditech, etc.
• Microsoft Office (Excel, Word, PowerPoint) for documentation and reporting
• Data analysis tools (SPSS, etc for research purpose)

Member of American Society for Microbiology (ASM)

Member Australia and New Zealand biosafety Association (ABSANZ) .

Member of Pakistan Aqua culture and Fisheries Society (PAFS) Member of Pakistan Society for Microbiology (PSM)

Member of Biological Safety Association of Pakistan (BSAP)

Selected in a US state project” Developing Next Generation of Female Environmentalist “in

Selected In “women leadership development Project of HESSA” as Senior Faculty Member in

Win Big idea design competition for Women Empowerment in Teachers category - April 2022

 Selected for Advance Training Course to be held in Malaysia for Bio Risk Management by CRDF Global in Nov 2017.

Travel award from CRDF Global to participate in ABSANZ 7th Annual General Meeting Conference held in Australia for poster presentation in Oct 2017.

 Selected as Mentee in a Mentorship Exchange Program by CRDF Global 2016 for Bio-Safety & Bio-Security.

Travel award from Health Security Partners (HSP) to participate America Society of Microbiology (ASM) Bio-threat and Bio-terrorism Conference at Washington DC to present poster in Jan 2017.

Travel award by Health Security Partners (HSP) to participate in America Society of Microbiology (ASM) General Meeting Conference for poster presentation in June 2016.

Travel award from America Society of Microbiology (ASM) to participate in the “Managing Biological Assets Responsibility” at the Sofitel Downtown, Dubai organized by America Society of Microbiology (ASM) in partnership with Behavioral-Based Improvement Solutions from Oct 28 to Oct 30, 2015.

· Travel award from Bio Safety Engagement Programme (BEP) to participate in America Society of Microbiology (ASM) general meeting conference for poster presentation in 2015.

· Travel award from America Society of Microbiology (ASM) to participate in the Asia-Pacific Biosafety Association (A-PBA) Conference “Biosafety & Biosecurity: Collective Partnership towards One World One Health”, at Pullman Bangkok King Power Hotel, Bangkok, Thailand organized by Asia-Pacific Biosafety Association (A-PBA) and the Biosafety Association of Thailand (BAT) from Nov 03 to Nov 06, 2014.

· Travel award from America Society of Microbiology (ASM) to participate in the “Biosafety Officer and Program Management” at the JW Marriott Hotel, Dubai organized by America Society of Microbiology (ASM) in partnership with Behavioral-Based Improvement Solutions from Oct 28 to Oct 30, 2014.

· Training award from the U.S. Dept. of Agriculture, Foreign Agricultural Service (USDA) to participate in “Certified Professional Instructor” program delivered by University of Idaho in collaboration with the University if Agriculture, Faisalabad – Pakistan from 11-16 July 2011.

· Travel award from U.S. Department of State to participate in the “Biosafety and Security Training” at the JW Marriott Hotel and National Center for Genetic Engineering and Biotechnology (BioTec). Organized by the U.S. Department of State's Biosecurity Engagement Program (BEP), Thailand- Bangkok from June 21-June 28, 2008.

Risk taker , leadership ,Capacity building ,Advocacy in women in STEM, Resilience, Mentor always love to work Cross cultural setting

1. Prof. Dr. Shahana Urooj Kazmi, Head - Immunology and Infectious Diseases Research Laboratory, Department of Microbiology, University of Karachi- Karachi-75270, Pakistan

Phone: Laboratory: 92 21 9261389 (direct), 9261300 ext:2358 Fax: 92 21 92610 Email: shahanaurooj@yahoo.com, shahanaurooj@uok.edu.pk

2. Dr Barbra Rasco Senior Provost University of Wyoming

509-990-7361. Work email is brasco@uwyo.edu.

3. Susan Carvalho |  secarvalho@ua.edu Associate Provost & Dean Graduate School  The University of Alabama
Ste. 100 Rose Administration Bldg. Box 0118 Tuscaloosa, AL 35487 Phone 205-348-8280 | Mobile 859-618-4399

Work as volunteer in Saudi field hospital at Mansehra for the rehabilitation of the effecties of earth quick as MICROBIOLOGIST with expert team of Pathologist and Microbiologist.

Conducted biosafety and bio security training as community service (more than 50 trainings and workshops among healthcare workers, Research students and trainees and M.Phil. students.

1 Paper Title:Activity of Curcumin against Drug-Resistant Gram-Negative Pathogens in Skin Infections Author(s) Name:1. Asim Gamaryani,Title of journal: indus journal of bioscience research2025 Date:2025-01-15

2 Paper Title:PPHARMACOGNOSTIC STUDIES OF ELAEAGNUS ANGUSTIFOLIA L. FROM BOI, DISTRICT ABBOTTABADAuthor(s) Name:Aymin Laraib Syed Nain Taara BukhariTitle of journal: Journal of Population Therapeutics Clinical Pharmacology Date:2024-02-28

3 Paper Title:THE INFLUENCE OF VARIOUS RISK FACTORS ON THE CORRELATION BETWEEN HPV INFECTION AND THE ADVANCEMENT OF CERVICAL CARCINOMAAuthor(s) Name:Bukhari, N.T.,Title of journal: Biological and Clinical Sciences Research Journal Date:2024-05-25

4 Paper Title:SYNERGISTIC EFFECT OF SILVER NANOPARTICLES AND ANTIBIOTICS AGAINST METHICILLIN RESISTANCE STAPHYLOCOCCUS AUREUSAuthor(s) Name:Nain Taara BUKHARITitle of journal: Journal of Population Therapeutics and Clinical Pharmacology Date:2024-06-05

5 Paper Title:SYNERGISTIC BIOGENIC SYNTHESIS OF ZINC NANOPARTICLES BY PROBIOTICS SACCHAROMYCES CEREVISIAE AND LACTOBACILLUS FERMENTUM FOR FARM ANIMAL HEALTH AND NUTRITIONAuthor(s) Name:Nain Taara BUKHARITitle of journal: Journal of Population Therapeutics and Clinical Pharmacology Date:2024-06-05

6 Paper Title:MOLECULAR CHARACTERIZATION OF ACINETOBACTER BAUMANNII AND METHICILLIN RESISTANT STAPHYLOCOCCI FROM SEPTICEMIA PATIENTS IN PESHAWARAuthor(s) Name:Nain Taara BUKHARITitle of journal: Journal of Population Therapeutics and Clinical Pharmacology Date:2024-03-30

7 Paper Title:ANTAGONISTIC ACTIVITY OF LACTOBACILLUS AND BIFIDOBACTERIUM ISOLATED FROM YOGURT AGAINST PATHOGENIC ENTERIC BACTERIAAuthor(s) Name:Nain Taara BUKHARITitle of journal: Journal of Population Therapeutics and Clinical Pharmacology Date:2024-03-11

8 Paper Title:Formulation of Nano-fertilizer and Phytochemical Screening of orange peel were utilized to enhance the growth of Vigna radiataAuthor(s) Name:Nain Taara BUKHARITitle of journal: International Journal of Phytopathology Date:2024-02-02

9 Paper Title:EVALUATION OF ANTIBACTERIAL ACTIVITY OF PIPER NIGRUM AND CUMINUM CYMINUM EXTRACTS ON BACTERIAL ISOLATES FROM THROAT INFECTIONAuthor(s) Name:Nain Taara BUKHARITitle of journal: Journal of Population Therapeutics and Clinical Pharmacology Date:2023-12-28

10 Paper Title:Exploring the Antibacterial, Antifungal, and Anti-Termite Efficacy of Undoped and Copper-Doped ZnO Nanoparticles: Insights into Mutagenesis and Cytotoxicity in 3T3 Cell LinAuthor(s) Name:Nain Taara BUKHARITitle of journal: Journal of Biological Regulators and Homeostatic Agents Date:2023-12-27

11 Paper Title:Exploring the Potential of Castor Oil for Effective Management of Seed-Borne Fungi in Zea maysAuthor(s) Name:Nain Taara BUKHARITitle of journal: Pakistan Journal of Weed Science Research Date:2023-12-27

12 Paper Title:ISOLATION AND CHARACTERIZATION OF PLANT GROWTH PROMOTING BACTERIA (PGPR) FROM RHIZOSPHERE OF OKRAAuthor(s) Name:Nain Taara BUKHARITitle of journal: Journal of Population Therapeutics and Clinical Pharmacology Date:2023-10-30

13 Paper Title:PHYTOSYNTHESIS, CHARACTERIZATION AND PHARMACOLOGICAL INVESTIGATION OF GOLD NANOPARTICLES USING LEAVES OF ACACIA MODESTA WALLAuthor(s) Name:Nain Taara BUKHARITitle of journal: Journal of Population Therapeutics and Clinical Pharmacology Date:2023-10-05

14 Paper Title:Chitosan improves the efficacy of gentamicin against MDR and biofilm-forming URO pathogenic E. coliAuthor(s) Name:Nain Taara BUKHARITitle of journal: International Journal of Health Sciences Date:2023-09-20

15 Paper Title:Functional genomics analysis of Leptin-Melanocortin system genes reveals candidate genes associated rapid growth and high carcass yield in sheepAuthor(s) Name:Nain Taara BUKHARITitle of journal: Journal of King Saud University - Science Date:2023-08-14

16 Paper Title:Toxicological effects of zinc oxide nanoparticles on hemato-biochemical profile of common carp (Cyprinus carpio)Author(s) Name:Nain Taara BUKHARITitle of journal: Journal of King Saud University - Science Date:2023-08-05

17 Paper Title:Prevalence and Antimicrobial Susceptibility Pattern of E.Coli Isolated From Raw Meat Samples in the Local Markets of PeshawarAuthor(s) Name:Nain Taara BUKHARITitle of journal: Tobacco Regulatory Science Date:2023-08-03

18 Paper Title:Antibacterial activity of Rhizopus specie isolated from rhizosphere of Mentha PiperitaAuthor(s) Name:Nain Taara BUKHARITitle of journal: International Journal of Health Sciences Date:2023-04-25

19 Paper Title:Seroprevalence And Molecular Detection Of Hepatitis B Virus (Hbv) And Hepatitis C Virus (Hcv) In Peshawar District, Khyber Pakhtunkhwa, PakistanAuthor(s) Name:Nain Taara BUKHARITitle of journal: journal of pharmaceutical negative results Date:2023-04-14

20 Paper Title:Anti-Bacterial And Anti-Fungal Activity Of Wild Plant Trillium Undulatum Against Multi-Drug Resistant PathogensAuthor(s) Name:Nain Taara BUKHARITitle of journal: Journal of PharmaceuticalNegative Results Date:2023-07-09

21 Paper Title:Prevelance DistributionAnd Risk Factors Of Hard Ticks In Large Ruminants Of District Gujrat,Punjab,PakistanAuthor(s) Name:Nain Taara BUKHARITitle of journal: Journal of pharmaceutical negative results Date:2023-07-05

22 Paper Title:Co-Heritability of Maturity and Yield Associated Traits In Mungbean (Vigna radiata. L.)Author(s) Name:Nain Taara BUKHARITitle of journal: Journal of Surveys in fishiries Science Date:2023-04-20

23 Paper Title:UNVEILING ANTIFUNGAL PROTEINS FROM TRAMETES VERSICOLOR AS BIO-PESTICIDE TO INHIBIT ALTERNARIA SOLANIAuthor(s) Name:Nain Taara BUKHARITitle of journal: Biological and Clinical Sciences Research Journal Date:2023-01-20

24 Paper Title:Degradation of fruits layers by enzymatic activity of fungiAuthor(s) Name:Nain Taara BUKHARITitle of journal: Pakistan journalof botany Date:2022-08-15

25 Paper Title:antimicrobial potential of ethanolic leaf extracts of parkinsonia aculeate using response surface methodologyAuthor(s) Name:Nain Taara BUKHARITitle of journal: Biological and Clinical Sciences Research Journal Date:2022-12-14

26 Paper Title:Recorganizing the resistance pattern of microorganisms in the subjects admitted in the icu ÂA prospective Observational studyAuthor(s) Name:Nain Taara BUKHARITitle of journal: E Cronicon Date:2018-05-11

27 Paper Title:Prevelance and control of multidrug resistance nosocomiaal pathogens isolated from hospital wards Author(s) Name:Nain Taara BUKHARITitle of journal: Microbiology research Journal international Date:2019-11-02

28 Paper Title:Incidence of Post-Harvest Fungal Rot of Some Vegetables in Swabi, Khyber Pakhtunkhwa PakistanAuthor(s) Name:Nain Taara BUKHARITitle of journal: Pakistan Journal of Agricultural Research Date:2021-07-13

29 Paper Title:Prevalence of ESBL producing MDR E. coli and Klebsiellae pneumonae from clinical isolates of nosocomial hospital acquired infectionAuthor(s) Name:Nain Taara BUKHARITitle of journal: GSC Biological and Pharmaceutical Sciences ISSN 2581-3250 Date:2020-05-16

30 Paper Title:A study of enteropathogenic E .coli (EPEC) and the contributing risk factors in diarrheal patients under fiveAuthor(s) Name:Nain Taara BUKHARITitle of journal: World Journal of Advanced Research and Reviews Date:2020-08-29

31 Paper Title:Inevitability of an Enhanced Monitoring Strategy to Reduce Water Borne Illness Combining Indicators of Sanitary Protection and Measuring Water QualityAuthor(s) Name:Nain Taara BUKHARITitle of journal: International Journal of Clinical Medicine ISSN Date:2016-09-11

32 Paper Title:Exploring the Antimicrobial Potential of Azadirachta Indica Against Pseudomonas Aeruginosa a Natural Approach to Combat Drug ResistanceAuthor(s) Name:Nain Taara BUKHARITitle of journal: indus journal of bioscience research2025 Date:2025-02-24

33 Paper Title:Talcs And Beneficial Bacteria Teaming Up To Shield Sunflowers From Root RotAuthor(s) Name:Nain Taara BUKHARITitle of journal: Journal of Pharmaceutical Negative Results Date:2023-07-22

34 Paper Title:Prevalence, Distribution And Risk Factors Of Hard Ticks In Large Ruminants Of District Gujrat, Punjab, PakistanAuthor(s) Name:Nain Taara BUKHARITitle of journal: Journal of Pharmaceutical Negative Results Date:2022-07-14

36 Paper Title:Investigating Meca Gene Expression And Its Molecular Impact On Methicillin Resistance Instaphylococcus AureusAuthor(s) Name:NainTaaraBukhari1,Title of journal: Journal of Medical and Health Science Review Date:2025-06-15