Cell proliferation assay with target chemical compounds, following fluorescence analysis.
BSc Project (Seddon's Lab)
UCL Institue of Immunity and Transplantation
London, China
2026.01 - 2026.05
Constructed IKK1/2-deficient mice models to assess whether the graded loss of IKK1 and IKK2 affects B cell population across the spleen, lymph nodes and bone marrow.
Processed spleen, lymph nodes, bone marrow samples to prepare single-cell suspensions for flow cytometry.
Performed cell surface staining with fluorescently labelled antibodies to characterise immune cell populations by spectral flow cytometry.
Summer Research Student
Peking University-Changping Laboratory
Beijing, China
2025.07 - 2025.09
Encapsulated mRNA into lipid nanoparticles using nano formulation machine, ready for subsequent administration into mice for vivo studies.
Isolated genomic and plasmid DNA via magnetic bead adsorption, followed by PCR amplification of target sequences.
Performed pseudo-virus neutralisation assays to evaluate the neutralising activity of antibody samples against SARS-CoV-2 pseudo-viruses.
Protein and antibody purification using both magnetic bead-based separation and resin-based column chromatography.
Performed Western blot to validate protein purity, Southern blot to confirm the length of amplified DNA strand.