KAYLEIGH THOMSON

• Operating equipment and supporting/executing validation activities, calibration of equipment and performance of daily equipment checks
• Adhering to cGMP standards and standard operating procedures (SOP)
• Assisting Manufacturing Biotechnologists during cell therapy processing e.g. performing cell count and viability assays, CD34+ cell isolation
• Completion and review of quality-related documentation including batch production records, training records and logbooks
• Escalate and report any deviations to materials, facilities, processes or procedures to the supervising team

• Conducting in-depth research on medical and scientific topics to create high-quality, engaging content for a lay audience to increase accessibility of scientific knowledge.

Supervised lab-based dissertation project: Investigating the molecular mechanisms of genetic predisposition to corneal thinning.

• Extensive cell and tissue culture experience
• Generation of cell-derived matrices for collagen immunofluorescence staining, imaging and analysis in wild-type and mutant genotypes
• RNA extraction and cDNA synthesis
• PCR, RT-PCR and RT-qPCR
• Preparation of cell and tissue lysates to perform Bradford assays
• Western blotting on cell lysates to detect protein expression
• Gateway cloning of regulatory regions and transfections with plasmids via neon electroporation
• Dual-luciferase assays and analysis
• Presentations of aims, methods, findings and conclusions
• Data analysis using software such as ImageJ and Excel

• Providing lively customer service with a friendly and professional demeanour, successfully managing high-volume periods with efficiency and professionalism
• Preparing and serving a variety of beverages, both alcoholic and non-alcoholic, and assisting in the restocking of bar supplies
• Excellent communication skills, attention to detail, and the ability to work effectively under pressure and solve queries
• Increasing sales through effective upselling techniques and product knowledge

• Assisting with mathematics and English to children aged 4-17 years old during the COVID-19 pandemic
• Regular evaluation of student progress and tailored constructive feedback to support academic growth
• Complying with COVID-19 regulations and safety procedures

• Operating tills and handling cash
• Solving customer enquiries efficiently
• Maintaining store appearance, replenishing stock and merchandising