Joshua Kariyil Jolly

An experienced professional with expertise in molecular biology techniques, laboratory management, and bioinformatics proficiency. Talented at adhering to GLP standards and implementing quality control techniques to ensure accuracy and reliability in research. Committed to advancing scientific knowledge as well as a team player with strong communication and independent working skills. I am dedicated to ensuring high standards and contributing to company's mission of deliver expectational services.

The recent project was on Utilizing synthetic bacterial biosensors to combat antimicrobial resistance (AMR) under Dr. Rahmi Yusef's supervision in De Montfort University. The project mainly deals with biosensors that are aromatic tunned to modulate signal output from two-component signaling circuits in a specific stimulus-dependent manner. The project utilized techniques such as fluorescent spectrophotometry, Gel Electrophoresis, and Western blotting. I am trained in primer designing, bacterial genetic engineering, and biosensor construction. I have experience in plasmid isolation by mini-prep, restriction digestion, ligation, and bacterial transformation using electroporation and heat shock techniques. I am skilled in fluorescent protein-based biosensor analysis, including signal output quantification using fluorescent spectrophotometry and plate reader assays. Additionally, I have expertise in protein expression analysis by Western blot, quantification using ImageJ and bacterial antimicrobial resistance screening. I have also worked with fluorescent tagging (CFP/YFP), SDS-PAGE, and microbiological techniques, contributing to novel two-component signaling system-based biosensor development for antimicrobial resistance detection.

I had worked on a project like Biodegradation of textile azo dyes using bacterial strains isolated from textile effluent and improvement of seedling growth in my time as Lab assistant and A review on isolation, identification of Bacillus and antimicrobial activity detection in my bachelor's dissertation. I was trained for the microbial isolation and identification, specifically isolating Bacillus species from agricultural soil and assessing their antimicrobial activity using special agar diffusion assays. I have experience in screening for novel antimicrobial compounds from bacterial isolates with a high enough potential for combating antibiotic-resistant pathogens. Moreover, I am versed in biodegradation studies, the bacterial strains isolated from textile effluent were used for the degradation of textile azo dyes and thereby improving the seedling growth. My competence lies in spectrophotometric analysis for dye decolorization, bioremediation techniques, and nontraditional source for the antimicrobial discovery.

Available upon request