Joel Varghese
Wolverhampton,West Midlands
Summary
Biomedical science graduate with hands-on experience in mammalian cell cultures and molecular techniques. Skilled in cell culture (including adherent, suspension, and hypoxic conditions), molecular techniques including PCR, Western blotting, stable transfection using CRISPR-Cas9, imaging techniques, flow cytometry, and ELISA. Proven track record of managing and executing complex lab experiments, and contributing to collaborative research projects. Passionate about learning liquid handling automation, NGS library preparation, and impactful data interpretation. Strong communicator with experience presenting findings, and supporting cross-functional lab operations.
Overview
7
7
years of professional experience
1
1
Certification
Work History
Phlebotomist
NHS Lewisham and Greenwich
04.2025 - 04.2025
- Obtained a variety of blood samples through venepuncture or capillary puncture using syringes, butterfly needles, and straight needles.
- Promoted blood specimen integrity for laboratory testing using accurate selection of collection tubes and correct technique.
- Interacted with patients to gather and record data prior to blood draws.
- Maintained a clean, safe, and organized work environment.
- Performed venipuncture and other blood collection procedures according to established standards.
MPhil Researcher
University of Wolverhampton
06.2022 - 03.2025
- Led research on inhibitory effects of secreted proteins on macrophages in glioblastoma, utilizing tumor-immune interaction and macrophage phenotype analysis.
- Conducted detailed experiments to explore macrophage inhibitory protein expression with cell culture techniques (adherent & detached) and flow cytometry for cell surface markers.
- Applied immune assays such as Phagocytosis using fluorescent microscopy and ELISA, demonstrating comprehensive knowledge of immunological processes.
- Mentored and guided 4 master's students in project design and scientific methodologies.
- Delivered research presentations at internal seminars, showcasing communication and presentation skills.
- Managed inventory, resource allocation, and data management to ensure accurate and efficient research documentation.
International Exchange Student
University of Wolverhampton
01.2020 - 05.2020
- Collaborated on investigating drug resistance mechanisms in pancreatic ductal adenocarcinoma.
- Gained hands-on experience in cell culture, PCR assays (RT-PCR, qPCR), Western blotting, flow cytometry, and stable transfection using CRISPR-Cas9 for knockout.
- Conducted hypoxia cell culture and cell viability assays to study cancer therapeutic responses.
- Mentored 2 undergraduate students, providing guidance in project design and scientific methodologies.
Student Researcher
VIT University
07.2018 - 11.2019
- Conducted an independent research project aimed at identifying fibrinolytic enzymes from rhizosphere soil bacteria for potential therapeutic applications in blood clot dissolution.
- Performed microbiological techniques including aseptic handling, Gram staining, serial dilution, plating methods, and bacterial enumeration for isolating and characterizing soil microorganisms.
- Executed biochemical and microbiological assays such as minimum inhibitory concentration testing and protein purification to evaluate enzymatic activity and antimicrobial properties.
- Cultured and maintained microbial strains including E. coli, Bacillus spp., and Saccharomyces cerevisiae under controlled laboratory conditions to support experimental reproducibility.
- Gained foundational experience in experimental design, data interpretation, and biological assay development relevant to translational microbiology.
Education
Master of Philosophy - Biomedical Sciences
University of Wolverhampton
Wolverhampton03.2025
Master of Science - Applied Microbiology
VIT University
India04.2020
Bachelor of Science - Microbiology
Presentation College of Applied Sciences
India03.2018
Skills
- Cell and Molecular Biology Techniques
- RT-PCR
- QPCR
- Western blotting
- Flow cytometry
- ELISA
- Phagocytosis assays
- CRISPR-Cas9
- Viability assays
- Hypoxic culture systems
- Experimental Design
- Data Analysis
- GraphPad Prism
- MS Office
- Laboratory Management
- Project Management
- Hazardous Waste Management
- Scientific Communication
- Collaboration
- Research Documentation
- Mentoring
Certification
- Phlebotomy Training from NHS Lewisham and Greenwich
Timeline
Phlebotomist
NHS Lewisham and Greenwich
04.2025 - 04.2025
MPhil Researcher
University of Wolverhampton
06.2022 - 03.2025
International Exchange Student
University of Wolverhampton
01.2020 - 05.2020
Student Researcher
VIT University
07.2018 - 11.2019
Master of Philosophy - Biomedical Sciences
University of Wolverhampton
Master of Science - Applied Microbiology
VIT University
Bachelor of Science - Microbiology
Presentation College of Applied Sciences
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