James Fleming

Key Experiences:

• Designed and optimized RT-qPCR assays for gene expression analysis.
• Collaborated with flow cytometry experts to design a complex flow cytometry panel.
• Refined process parameters to enhance transgene and protein expression.
• Designed landing pad constructs for directed integration of transgenes.
• Identified target genes for KO studies.

Key Responsibilities

• Collaborated with the Cell therapy Innovation team to develop a hypo-immune iPSC cell line
• Discussed and presented innovative ideas to overcome limitations
• Performed literature reviews to guide project planning and assay designs
• Presented experimental results and assay designs to the Cell Therapy Innovation Team
• Documentation of experimental activities and results using ELN/LIMS systems

Key Experiences

• Generated the Revvity ADCC+ CHO cell line using AAV gene-editing technology
• Performed literature reviews on regulatory vector elements to optimise antibody expression
• Applied advanced cloning techniques to construct recombinant protein expression vectors
• Achieved a 50% increase in biotherapeutic productivity through collaborative research
• Optimisation of feeding strategies for fed-batch overgrow cultures, maintaining optimal cell culture environments by quantifying metabolite values
• Enhanced the transfection process for transposon technology, leading to improved biotherapeutic expression and enhanced pool recovery
• Presented findings at BioProNET2 2023, securing 3rd place for scientific achievement
• Led cell line development projects from pool production to clonal cell line generation, ensuring monoclonality and genetic stability
• Designed and optimised ddPCR and qPCR assays for the characterization and evaluation of cell lines
• Gained extensive experience in BSL-2 laboratories, including HEK-293 cell culture, HEK-293 transfection, AAV transduction, quantification, and purification

Key Responsibilities

• Delivered accurate, high-quality data under high-pressure deadlines
• Communicated results and presented data to the Bioproduction team and wider Bioproduction business unit
• Presented innovative ideas and solutions to complex challenges in quarterly innovation meetings
• Collaborated with the Revvity operational excellence team over three years to implement 5S standards across multiple laboratories, conducting regular audits to maintain high standards and improve consumable usage
• Composed, reviewed and updated standard operating procedures (SOPs) to ensure compliance with good laboratory practices (GLP)
• Authored experimental reports using Graph Pad Prism for statistical analysis
• Mentored junior scientists on techniques and data analysis
• Ensured rigorous documentation for full traceability of reagents and consumables for product development, capturing all data in ELN systems