Jack Buckley

Current MSc by research student eager to gain more experience in a laboratory setting. I am hoping to acquire skills in bacterial genomics and sequencing, as well as proteomics and bioinformatics. I have developed a passion for microbiology and the treatment of bacterial infections and aim to produce quality research in this field.

• Microbiological Assays – Experience with numerous assays and techniques such as disc diffusion, tine-kill kinetics, multiple antibiofilm procedures, SEM imaging preparation, Gram staining, wound modelling. These assays have facilitated me to become competent with using autoclaves, pipettes, digital callipers, CDC biofilm reactors, Sonicators. I am also comfortable with the common necessities of a microbiology laboratory, such as, pouring agar plates, producing streak plates, creating bacterial lawns, serial dilutions, producing cultures of a specific optical density.
• RT-qPCR – DNA and RNA extractions of both bacterial and viral samples. Experience with reverse transcriptase reaction, use of the thermocycler, operating liquid handling system/automated pipetting robot, interpretation of data using melt curves for both diagnostic work and research. As well as working in a class II biohazard hood with covid-19 samples.
• Cell Biology– Experience culturing HEP G2 and HCAT cell lines and performing cytotoxicity evaluation of various compounds.
• Chemistry – Phytochemical profiling of natural product extracts, giving me experience in making reagents and measuring/altering pH. LC-MS and interpretation will be completed prior to completion of MSc.