HARSH PANDEY
Oxford,United Kingdom
Summary
Accomplished professional with expertise in molecular and analytical techniques, including qPCR, RT-PCR, ELISA, and Western blotting. Proficient in FTIR spectroscopy, HPLC, flow cytometry, and fluorescence microscopy. Skilled in primer design and validation, with extensive experience using NCBI BLAST and UCSC Genome Browser. Committed to advancing scientific research through innovative methodologies and precise data analysis.
Overview
2
2
years of professional experience
4
4
years of post-secondary education
Work history
Laboratory technician
Newrotex Pvt Ltd
Oxford
2024.10 - 2026.01
- GMP Biomanufacturing & Process Optimization: Maintain live spider colonies and optimized spider-silk extraction across 200+ specimens, improving reproducibility by 25% and supporting consistent in-vitro testing.
- Analytical characterization and data interpretation: Conducted FTIR spectroscopy to quantify α-helix and β-sheet content, correlating structural profiles with material stability and improving product consistency by 20%.
- Systematic Evidence Synthesis & Literature Analysis: Conducted structured review of scientific literature and internal experimental data to guide biomaterial process optimization, collating and summarizing findings from 50+ studies to inform experimental design and ensure reproducibility.
- Equipment qualification and laboratory operations: Performed IQ/OQ of manufacturing systems (CO2 incubators, biosafety cabinets, 3D bioprinters) and managed ISO Class 5 cleanroom operations, ensuring contamination-free conditions and system reliability for reproducible experimental results.
- Training & Mentorship: Provided technical guidance to interns on analytical, molecular techniques, aseptic practices, and GMP documentation standards.
Research assistant
Nottingham Trent University
Nottingham
2023.04 - 2023.06
- Functional in-vitro modelling: Investigated the effects of gut microbiota–derived quorum-sensing molecules on SH-SY5Y and N2a cells using MTT viability assays, immunofluorescence microscopy, and IncuCyte live-cell imaging, identifying concentration-dependent functional responses.
- Epigenetics Research (ChIP-qPCR): Performed Chromatin Immunoprecipitation with MNase-digested chromatin and qPCR analysis to quantify histone H3/H3K9 methylation, demonstrating reliable immunoprecipitation efficiency and chromatin profiling.
- Immune-related signaling exposure: Conducted a focused pilot analysis of stress- and inflammation-associated cellular responses in SH-SY5Y cells using ELISA-based cytokine detection and microscopy to assess functional cellular changes.
- Molecular Diagnostics & Bioinformatics: Designed, validated, and optimized PCR primers for lukF-PV differentiation using sequence analysis tools (NCBI BLAST, CARD), demonstrating assay development capabilities applicable to diagnostic product development.
Research and Development Intern
Raks Pharmaceutical PVT.LTD
Gujarat
2022.06 - 2022.12
- Human in-vitro cell culture: Maintained HEK293 and CHO-K1 mammalian cell lines under GMP conditions for drug cytotoxicity assays, optimizing cell growth and viability to support production-scale testing in 5L bioreactors.
- Functional cell-based assays: Conducted multi-well plate-based cytotoxicity and viability assays, generating reproducible functional datasets applicable to immune modulation, cell activation, and therapeutic screening studies.
- Analytical Characterization: Applied molecular and analytical techniques (HPLC, MS) to characterize bioactive compounds with potential immunomodulatory effects, ensuring experimental rigor and reproducibility.
- Experimental standardization and reporting: Compiled structured assay protocols, validation summaries, and technical reports, reinforcing reproducibility, data integrity, and research readiness.
Education
Master of Science - Biotechnology
Nottingham Trent University
Nottingham, UK 2023.01 - 2024.02
Bachelor of Science - Biotechnology
Devi Ahilya University
Indore, India 2019.01 - 2022.01
Skills
- Molecular and analytical techniques
- qPCR and RT-PCR
- ELISA and Western blotting
- FTIR spectroscopy
- HPLC and flow cytometry
- Fluorescence microscopy
- Primer design and validation
- NCBI BLAST and UCSC Genome Browser
Accomplishments
- Contributed to company-wide ISO 13485 certification - (November 2025)
- Developed QMS documentation, authored SOPs, and supported GMP process validation to enhance regulatory compliance and audit readiness.
- Cytiva Regulatory: Good Documentation Practices (training completed) - (October 2025)
- Training in GDP principles, documentation control, and data integrity in GMP-regulated environments.
Work availability
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
morning
afternoon
evening
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Timeline
Laboratory technician
Newrotex Pvt Ltd
2024.10 - 2026.01
Research assistant
Nottingham Trent University
2023.04 - 2023.06
Master of Science - Biotechnology
Nottingham Trent University
2023.01 - 2024.02
Research and Development Intern
Raks Pharmaceutical PVT.LTD
2022.06 - 2022.12
Bachelor of Science - Biotechnology
Devi Ahilya University
2019.01 - 2022.01