Years of research experience
Gautham Ramakrishnan
Researcher | Problem Solver
Moore
Summary
Motivated scientist and critical thinker with a strong background in immunology, cell biology, and molecular biology. Interested in pursuing a research career in the field of Immunology.
Overview
6
6
10
10
years of post-secondary education
Work History
Graduate Research Assistant
The University of Oklahoma Health Sciences Center
Oklahoma City, OK
07.2018 - Current
- Carried out hypothesis driven research on the role of SHIP phosphatases in the regulation of TREM2-mediated microglial function in the context of Alzheimer's disease.
- Independently planned and performed experiments and data analyses for my project while contributing intellectually towards other projects during lab meetings.
- Worked in a small lab where I learned, optimized, and executed literature-derived experimental protocols from scratch and established them in the laboratory.
- Contributed intellectually to campus-wide research symposia and smaller-scale groups like the Myeloid Study Club at the University.
- Was the head of the Microbiology and Immunology Departmental Retreat organizing committee in 2022.
- Received the Bill and Charlene Wilson Award for Scientific Innovation in 2021 and a $1000 travel award at the Graduate Research Symposium on campus.
Research Assistant
Renal Genetics Lab, St. John’s Research Institute
Bangalore
03.2017 - 06.2018
- Studied monocyte driven inflammation and bone mineralization in pediatric chronic kidney disease (CKD).
- Established a new multi-color flow cytometry panel for functional characterization of monocyte subsets in the lab.
- Was the sole employee in a new lab and learned to work independently to balance research responsibilities with management of lab inventory and orders, and training of new lab members.
- Involved in all aspects of research workflow from patient sample collection and processing to data generation, management, and presentation.
Drug Safety Associate Trainee
QuintilesIMS
Bangalore
08.2016 - 02.2017
- Generated and processed adverse event reports for multiple drugs.
- Worked on deadline-driven projects and developed great organizational and time management skills.
Teaching Assistant
SRM University
Chennai
09.2014 - 05.2016
- Provided hands-on training in microbiology and molecular biology to undergraduate students.
- Assisted teachers with classroom management and preparation of reagents and chemicals.
Education
Ph.D. - Microbiology and Immunology
The University of Oklahoma Health Sciences Center
Oklahoma City, OK, United States 07.2018 - Current
Master of Technology - Genetic Engineering
SRM University
Chennai, India 08.2014 - 05.2016
Bachelor of Engineering - Biotechnology
Visvesvaraya Technological University
Bangalore, India 09.2010 - 06.2014
Skills
Microbiology: Handling and cultivation (liquid and solid cultures) of aerobic and anaerobic bacteria, algae and fungi, bacterial transformation, and light microscopy
Molecular Biology and Biochemistry: PCRs, SDS-PAGE, Western blotting, measurement of cellular mitochondrial respiration and glycolysis using Seahorse XF analyzer
Immunology: Immunoprecipitation, ELISA, PBMC isolation from human blood, immunophenotyping, intracellular cytokine staining, and multi-parameter flow cytometry
Cell Biology: Mammalian cell culture (primary cells and multiple cell lines), cell differentiation, transfection, fluorescence microscopy, and cell signaling assays
Animal Handling: Management of mouse colonies, basic handling and dissections for organ harvesting and extraction of primary bone marrow cells in mice
Relevant Software: Microsoft Office, FlowJo, GraphPad Prism
Timeline
Ph.D. - Microbiology and Immunology
The University of Oklahoma Health Sciences Center
07.2018 - Current
Graduate Research Assistant
The University of Oklahoma Health Sciences Center
07.2018 - Current
Research Assistant
Renal Genetics Lab, St. John’s Research Institute
03.2017 - 06.2018
Drug Safety Associate Trainee
QuintilesIMS
08.2016 - 02.2017
Teaching Assistant
SRM University
09.2014 - 05.2016
Master of Technology - Genetic Engineering
SRM University
08.2014 - 05.2016
Bachelor of Engineering - Biotechnology
Visvesvaraya Technological University
09.2010 - 06.2014
Presentations
- Ramakrishnan GS, and Humphrey MB. "SHIP-2 inhibits human microglia-like cell function in a TREM2 independent manner". Oral presentation at IMMUNOLOGY 2022 organized by the American Association of Immunologists (AAI) at Portland, OR, USA.
- Ramakrishnan GS, and Humphrey MB. “The Role of SHIP phosphatases in human microglial like cell function”. Poster presentation at the Neuroscience Symposium (2022) at OUHSC.
- Ramakrishnan GS, and Humphrey MB. "SHIP inhibitors improve human microglial-like cell functions". Poster presentation at IMMUNOLOGY 2021 organized by the American Association of Immunologists (AAI).
- Ramakrishnan GS, Lal S, and Kolandaswamy A. Monocyte subsets – understanding key players in chronic kidney disease. Poster presentation at the International Congress of Cell Biology (2018) organized by the Center for Cell and Molecular Biology, India.
Publications
- Manali, K. M., Arunraj, R., Ramakrishnan, G. S., & Ramya, M. (2018). Development of sensitive and specific multiplex PCR method for the detection of microcystin producing cyanobacteria in spirulina food supplements. Food science and biotechnology, 28(2), 609–614. https://doi.org/10.1007/s10068-018-0476-0
- Gautham Subramaniam Ramakrishnan, Anwar Aliya Fathima & Mohandass Ramya. (2017). A rapid and efficient DNA extraction method suitable for marine macroalgae. 3 Biotech, 7(6), 364. https://doi.org/10.1007/s13205-017-0992-2
Competencies
- Extensive academic research experience and strong technical background in immunology and cell biology. Capable of independent design and execution of experiments.
- Articulate presentation of scientific data and excellent written and oral communication skills.
- Leadership skills acquired from organizing departmental events at OUHSC and working as a compere in cultural shows in college.
- Strong interpersonal skills gained from training and working with individuals from diverse backgrounds in different research environments.
Awards and Recognition
- Bill and Charlene Wilson Award for Scientific Innovation (2021)
- SRM - GATE assistantship for postgraduate studies (2014-2016)