Ganga Lakshmanan

Microbiologist

Medicity Multispeciality Laboratory

07.2022 - 07.2023

Bacterial Culture and Isolation: Performed streak plating, spread plating, and pour plating to isolate bacterial colonies
Used selective and differential media (e.g., MacConkey, Blood Agar, Mannitol Salt Agar) for bacterial identification
Maintained aseptic techniques for handling clinical and environmental samples
Bacterial Staining and Microscopy: Gram Staining for differentiating Gram-positive and Gram-negative bacteria
Acid-Fast Staining (Ziehl-Neelsen) for Mycobacterium species
Endospore Staining for detecting spore-forming bacteria (e.g., Bacillus, Clostridium)
Capsule Staining (India Ink/Nigrosin) for visualizing bacterial capsules
Used light microscopy to analyze stained slides and interpret bacterial morphology
Antibiotic Sensitivity Testing (AST): Performed Kirby-Bauer Disc Diffusion Method manually
Measured zones of inhibition using a ruler or caliper to determine bacterial susceptibility
Interpreted results based on CLSI guidelines to assess antibiotic resistance
Laboratory Management: Managed inventory and ordering of lab supplies, including culture media, reagents, and stains
Ensured proper storage and handling of bacterial cultures and chemicals to maintain quality and safety
Maintained laboratory cleanliness and biosafety protocols to prevent contamination
Laboratory Equipment Calibration and Maintenance: Performed calibration of laboratory equipment, including micropipettes, pH meters, and incubators, to ensure accuracy
Conducted routine quality control checks on reagents and instruments
Waste Disposal and Biosafety Compliance: Followed proper waste segregation and disposal methods for biological, chemical, and sharps waste
Adhered to biosafety and infection control protocols to minimize risks