Csaba Zoltán Kibédi Szabó
London,United Kingdom
Summary
Dedicated professional with expertise in Cryo-EM grid preparation, optimisation, and high-resolution imaging, complemented by proficiency in single particle analysis using CryoSPARC and RELION. Demonstrates exceptional skill in high-resolution Cryo-EM structure interpretation, alongside a strong foundation in protein expression and purification. Adept in conducting biochemical and biophysical assays and employing molecular biology techniques. Known for clear scientific communication, mentoring, and cross-functional teamwork, while being self-motivated and creative. Aspires to advance the field of structural biology through innovative research and collaboration.
Overview
12
12
years of professional experience
16
16
years of post-secondary education
Work history
Postdoctoral researcher
Institute of Cancer Research
London, United Kingdom
05.2022 - 05.2025
- Isolated and purified native spliceosomes and splicing-coupled polyadenylation complexes, stalled with small molecule inhibitors for mechanistic analysis via cryo-EM.
- Prepared and optimized cryo-EM grids for imaging on high-end microscopes (Titan Krios, Glacios), applied single-particle analysis workflows using cryoSPARC and RELION.
- Supervised a cryo-EM-driven fragment-based drug discovery project, interpreted high-resolution cryo-EM electron density maps.
- Independently initiated the integration of AlphaFold3 predictions to interpret cryo-EM electron density maps and guide functional hypotheses.
- Applied a suite of biochemical and biophysical assays, including in vitro splicing/polyadenylation, western blotting, mass photometry, gradient ultracentrifugation, and protein cross-linking MS.
- Mentored and trained colleagues in cryo-EM sample preparation, microscopy, and data processing.
- Presented research findings to cross-functional teams and scientific audiences.
- Collaborated with interdisciplinary teams including biochemists, biophysicist and cell biologist.
PhD Researcher
Institute of Cancer Research and the University of London
London, United Kingdom
10.2019 - 04.2022
- Purified multi-megadalton complexes involved in splicing and polyadenylation for structural studies.
- Prepared cryo-EM grids, imaged on Titan Krios and Glacios microscopes, followed by SPA using cryoSPARC and RELION.
- Performed a broad range of biochemical and biophysical assays, including in vitro transcription, mass photometry, gradient ultracentrifugation, immunoprecipitation, pull-down assays.
PhD Researcher
Max Planck Institute for Multidisciplinary Sciences and The University of Göttingen
Göttingen
09.2013 - 09.2019
- Expressed and purified a diverse range of proteins and protein complexes in E. coli and insect cell systems for downstream structural and functional analysis.
- Conducted protein crystallization trials and optimised grid preparation for negative stain and cryo-EM.
- Imaged samples on multiple platforms (F20, Titan Krios, Glacios) and analysed data using RELION and cryoSPARC.
- Applied a comprehensive set of biochemical and biophysical assays, including in vitro ubiquitination, SEC-MALS, thermal shift assays, gel filtration, pull-down.
- Used molecular biology techniques (cloning and site-directed mutagenesis) to generate constructs optimized for expression, stability, structural and functional studies.
- Contributed to successful grant application and peer-reviewed publication by compiling and presenting high-quality biochemical data.
Education
Doctor of Philosophy - Structural biology
University of London
09.2019 - 05.2022
PhD student - Structural biology
The University of Göttingen
09.2013 - 09.2019
Master of Science - Biochemistry
Şcoala Normală Superioară Bucureşti (SNSB)
10.2009 - 09.2011
Master of Science - Biochemical Engineering and Sustainable Biotechnologies
University POLITEHNICA of Bucharest
10.2009 - 02.2011
Bachelor of Science - Food Products Engineering
Sapientia Hungarian University of Transylvania
10.2005 - 07.2009
Skills
- Cryo-EM grid preparation, optimisation, and high-resolution imaging
- Single particle analysis with CryoSPARC and RELION
- High-resolution Cryo-EM structure interpretation
- Protein expression and purification
- Biochemical and biophysical assays
- Molecular biology techniques
- Clear scientific communication, mentoring, and cross-functional teamwork,
- Self-motivated and Creative
Custom
Organise and lead a regular institutional football social activity, fostering a positive and inclusive work environment. Volunteer structural biology curator for the Open Biology journal preprint review team.
Timeline
Postdoctoral researcher
Institute of Cancer Research
05.2022 - 05.2025
PhD Researcher
Institute of Cancer Research and the University of London
10.2019 - 04.2022
Doctor of Philosophy - Structural biology
University of London
09.2019 - 05.2022
PhD Researcher
Max Planck Institute for Multidisciplinary Sciences and The University of Göttingen
09.2013 - 09.2019
PhD student - Structural biology
The University of Göttingen
09.2013 - 09.2019
Master of Science - Biochemistry
Şcoala Normală Superioară Bucureşti (SNSB)
10.2009 - 09.2011
Master of Science - Biochemical Engineering and Sustainable Biotechnologies
University POLITEHNICA of Bucharest
10.2009 - 02.2011
Bachelor of Science - Food Products Engineering
Sapientia Hungarian University of Transylvania
10.2005 - 07.2009
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