Arjuna Sreenivasan
Manchester,MAN
Summary
Highly analytical and skilled bioinformatician/geneticist with a deep passion for research. Technically proficient individual with over 5 years of expertise in research, analysis, and reporting of complex clinical NGS and Chromosomal Microarray data for leading healthcare institutions in India. Proven ability to manage challenging research-oriented problems and come up with interesting solutions. Seeking a position to contribute knowledge of molecular biology and computer science in the field of Next Generation Sequencing/Microarray technology while facilitating professional growth.
Overview
5
5
years of professional experience
Work History
Senior Executive-Molecular Genetics
Lifecell International Private Ltd
05.2021 - 08.2022
- Designing Primers for detection of targeted variants for Sanger Sequencing
- Analysis of Sanger data and reporting for targeted variants for various genetic disorders
- Screening for variants using Sanger Sequencing for different Single Gene Disorders like Thalassemia, Sickle Cell Anaemia
- MCC detection by STR analysis using specific markers
- Training freshers and new joiners for primer designing, sanger analysis, and reporting
- Ensure report is getting released before assigned TAT and handling of team members ensuring their coordination
Senior Bioinformatician
Manipal Health Enterprises
10.2020 - 05.2021
- Self-motivated, with a strong sense of personal responsibility.
- Worked effectively in fast-paced environments.
- Skilled at working independently and collaboratively in a team environment.
- Proven ability to learn quickly and adapt to new situations.
- Database and server management and Validation and verification of clinical bioinformatics software
- Planning and management of different bioinformatics software deployment and continues improvements and updates of existing tools and pipelines
- Examining data and related bioinformatics processes to investigate and resolve difficult bioinformatics problems
- Processing and analyzing the Next Generation Sequencing ( NGS)(Ion S 5 platform) Whole Exome, Clinical Exome and Targeted panels, data and preparing concise presentations of computational results
- Performing robust data quality control, validation, and storage of genetic data generated
- Target identification by variant filtering, identification, and prioritization of biological data using multiple bioinformatics tools along with in-depth literature search for case-solving and reporting
- Equally capable of NGS variant filtering and prioritization using manual methods and commercially available software like Golden Helix
- Working closely with the clinicians to understand, solve and report the variant
- Designing and validation of new gene panels (AmpliSeq designer) targeted for specific diseases
- Primer designing, validation and analysis, and reporting for targeted variants using Sanger
- Planning, coordinating, and executing Chromosomal Microarray ( testing of CMA facility at the department
- Teaching, training, and monitoring students/ interns in the department
- Costing, planning, and coordinating with multiple teams for a new test launch and its execution
- Interacting from the department side with the company officials given the purchase of new software or products
Bioinformatician
Manipal Health Enterprises
09.2017 - 09.2020
- Self-motivated, with a strong sense of personal responsibility.
- Worked effectively in fast-paced environments.
- Skilled at working independently and collaboratively in a team environment.
- Proven ability to learn quickly and adapt to new situations.
- Database and server management and Validation and verification of clinical bioinformatics software
- Planning and management of different bioinformatics software deployment and continues improvements and updates of existing tools and pipelines
- Examining data and related bioinformatics processes to investigate and resolve difficult bioinformatics problems Workingking closely and communicating with the laboratory's clinical, scientific, and technical departments
- Processing and analyzing the Next Generation Sequencing ( NGS)(Ion S 5 platform) Whole Exome, Clinical Exome and Targeted panels, data and preparing concise presentations of computational results
- Performing robust data quality control, validation, and storage of the genetic data generated
- Target identification by variant filtering, identification, and prioritization of the biological data using multiple bioinformatics tools along with an in-depth literature search for case-solving
- Equally capable of NGS variant filtering and prioritization using manual methods and using commercially available software like Golden Helix
- Working closely with the clinicians to understand, solve and report the variant
- Designing and validation of new gene panels (AmpliSeq designer) targeted for specific diseases
- Primer designing, validation and analysis, and reporting for targeted variants using Sanger
Lab Technician- Bioinformatics Trainee
Department of Medical Genetics, Kasturba Hospital
06.2017 - 07.2017
- Learned new materials, processes, and programs quickly.
- Participated in on-the-job training, working closely with supervisors and coworkers and asking appropriate questions.
- Attended training courses to build understanding of processes, techniques, and industry.
- Supported departmental tasks to increase understanding of industry processes.
- Handling and analyzing NGS (Illumina platform) raw data using the Linux platform
- Running NGS raw data through the in-house bioinformatics pipeline to annotate data with
multiple levels of biological information which helps with case solving, target identification
and target prioritization - Continuous development of new computational techniques and integration of those techniques into current internal pipelines
- Communicate with researchers and clinicians about genomic data in clear and professional manner
Education
Master of Science - Medical Genetics
University of Chester
Chester, United Kingdom03.2023
Master of Science - Bioinformatcs
Manipal Academy of Higher Education
Manipal, Karnataka, India11.2015
Master of Science - Healthcare And Medical Engineering
University of Joseph Fourier
Grenoble, France07.2015
Bachelor of Science - Microbiology, Biochemistry, Botany
Mangalore University
Mangalore, India06.2013
Skills
Bioinformatics tools and Software:
- Agilent SureCall, Agilent CytoGenomics, Agilent SureSelect, Agilent
- Scan Control, Aglinet Feature Extraction, Ion Reporter, Ion AmpliSeq Designer, IGV, Limma
- Genevestigator, FastQC, FastX toolkit, Cutadapt, Bowtie, PyMOL, SwissPDB viewer, VMD and NAMD
- Autodesk molecule viewer, Swissmodel and modeller, Autodock vina, Tripos SYBYL-X, Glide (Schrodinger Suite), Desmond (Schrodinger Suite), GROMACS, Ingenuity Pathway Analysis, Golden Helix
- Databases: Ensembl genome browser, UCSC Genome Browser, dbSNP, gnomAD, ClinVar, Varsome
Programming Skills:
- Shell scripting, Python, Perl, R
Wet Lab Skills:
- PCR, Western blot and other molecular biology wetlab techniques
- Capable of Designing, standardizing and troubleshooting molecular biology experiments
Accomplishments
- Developed a database centric Bioinformatics Pipeline for proteomic analysis, 2014-15
CHU de Grenoble, IAB, Institut de Biologie et de Pathologie Plateforme Protéomique PROMETHEE Grenoble, France
Gained in depth knowledge in proteomics, mass spectrometry, java programming and DBMS - Identification of Protein from E coli Sample using Mass Spectrometry based proteomic analysis, 2015
CHU de Grenoble, IAB, Institut de Biologie et de Pathologie, Plateforme Protéomique PROMETHEE Grenoble, France
Gained in depth knowledge in molecular techniques like SDS PAGE, HPLC, Mass spectrometry - Festival of Genomics 2023 London
References
- Dr. Mitesh Shetty
HOD, Medical Genetics,
Manipal Hospitals, Bangalore
Phone: +91 9880923433
Email: mitesh@manipalhospitals.com
- Dr. Jayakrishna Jagannatham
Manager- Molecular Genetics
Lifecell International Private Limited
Phone: +91 9791126800
Email: jayakrishna.j@lifecell.in
Timeline
Senior Executive-Molecular Genetics
Lifecell International Private Ltd
05.2021 - 08.2022
Senior Bioinformatician
Manipal Health Enterprises
10.2020 - 05.2021
Bioinformatician
Manipal Health Enterprises
09.2017 - 09.2020
Lab Technician- Bioinformatics Trainee
Department of Medical Genetics, Kasturba Hospital
06.2017 - 07.2017
Master of Science - Medical Genetics
University of Chester
Master of Science - Bioinformatcs
Manipal Academy of Higher Education
Master of Science - Healthcare And Medical Engineering
University of Joseph Fourier
Bachelor of Science - Microbiology, Biochemistry, Botany
Mangalore University
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