Overview
Work history
Education
Skills
Prizes and Awards
Publications
Communications and Presentations
Professional Skills
Timeline
Generic

Agnieszka N. Urbanek

Sheffield

Overview

14
14
years of professional experience
9
9
years of post-secondary education

Work history

Postdoctoral Research Associate

University of Sheffield
01.2023 - 09.2025
  • SITraN, Neuroscience Department.
  • Project: Investigating protein aggregation in dementia.
  • This project aims to discover how Amyloid-beta aggregation and ApoE impact the development and progression of Alzheimer’s disease using iPSC-derived cortical neurons.


Research Assistant

University of Sheffield
01.2020 - 01.2023
  • SITraN, Neuroscience Department.
  • Project: Developing an AAV-based gene therapy for SPG4 Hereditary Spastic Paraplegia.
  • This project, in cooperation with SWANBIO, focused on developing an AAV-based gene therapy for HSP caused by mutations in the SPG4 gene using a mouse model.


Senior Research Technician

University of Sheffield
01.2017 - 01.2019
  • Department of Animal and Plant Sciences.
  • Project: Experimental adaptation and speciation in rotifers.
  • I developed protocols, coordinated all lab activities, and managed and trained other technicians and people associated with the project.
  • I was responsible for health and safety inspections, COOSH and risk assessment, and purchasing lab supplies.

Postdoctoral Research Associate

University of York
01.2015 - 01.2017
  • Biology Department.
  • Project: A combinatorial approach to enhance the production of monoclonal antibodies.
  • This work focused on enhancing successive steps that are potentially rate-limiting for mAb production, including transgene expression, mRNA translation, intracellular trafficking, post-translational modification, and secretion.

Postdoctoral Research Associate

University of Sheffield
01.2012 - 01.2015
  • Biomedical Science Department.
  • Project: Defining pathways that ensure unidirectionality of endocytosis.
  • I elucidated how the Las17/WASP protein is regulated, so its many interactions take place sequentially, and determined how this mechanism ensures the unidirectionality of endocytosis.


Education

PhD - Cell Biology

University of Sheffield
01.2008 - 01.2012

Master's and an engineer - undefined

West Pomeranian University of Technology (the University of Agriculture)
01.2001 - 01.2006

Skills

  • Molecular biology: DNA, plasmid DNA, RNA extraction, restriction digest, PCR, primer design, molecular cloning, mutagenesis, yeast genetics and techniques including recombination, gene knock-outs, gene tagging and mating, sequencing, genotyping
  • Biochemistry: HIS-, GST-tagged and untagged protein expression and purification, TAP-tag protein complexes purification, immunoprecipitation, co-immunoprecipitation, cell lysates, SDS-PAGE and Western blotting, lipid binding assays, and affinity purification of antibodies
  • Cell culture: mouse primary cortical neurons, iPSC-cortical nerons, HEK cells, passaging, cell lysate, cell fractionation, immuno-staining, cell transfection and transduction, microfluidic chambers
  • Microscopy: Light microscopy, Fluorescence microscopy, Live cell imaging, Fixed cell imaging, Single particle tracking
    Confocal microscopy
  • Animal work: Home office PIL A, B, C licence, schedule 1 techniques, mouse colony maintenance, Lab Tracks

Prizes and Awards

2025 - ARUK pumping grant -The role of Aβ trafficking in synaptic function: £5,907,12

2025 – travel grant for ADPD 2025 - £600 Guarantors of Brain Travel grant, £600 – SMPH travel fund

2024 – ARUK Yorkshire Network for a travel grant, £500

2016 – IBCarb travel fund - Challenges and Opportunities for Optimising Protein Glycosylation, Imperial College London - £100.

2014/2015, SURE – Sheffield Undergraduate research experience – £500.14 on reagents,

£2000 scholarship for an undergraduate student on the project awarded by the Wellcome Trust

2014 - Learned society travel fund–Dynamic Cell, Cambridge 2014 - £350

2011 - FEBS Youth Travel Fund for Actin-Based Motility-from Molecules to Model Organisms conference - £620

2009, Bristol, the 1-st prize for poster presentation at the Actin conference - £100.

Publications

  • Emily E. Prescott, Jiapeng Wei, Emma F. Garland, Agnieszka Urbanek, Francesco Capriglia, Willem H. Molenkamp, David P Rhodes, Louise Heywood, Aurelie Schwartzentruber, Elezabeth Stephen, Rachel Hughes, Oliver Bandmann, Mark O. Collins, Sara Linse, Axel Abelein, Jan Johansson, Michele Vendruscolo, J Robin Highly, Tuomas P.J. Knowles, Heather Mortiboys, Suman De, Secondary nucleation of α-Synuclein drives Mitochondria dysfunctions and Lewy body formation in Parkinson’s Disease, Molecular Cell, submitted
  • Urbanek A, Garland EF, Prescott EE, King MC, Olerinyova A, Wareing HE, et al., Molecular Determinants of Protein Pathogenicity at the Single-Aggregate Level, Adv Sci (Weinh), 2025, 12, 9, e2410229
  • Xia Z, Prescott EE, Urbanek A, Wareing HE, King MC, Olerinyova A, et al., Co-aggregation with Apolipoprotein E modulates the function of Amyloid-beta in Alzheimer's disease, Nat Commun, 2024, 15, 1, 4695
  • Rzepnikowska W, Flis K, Kaminska J, Grynberg M, Urbanek A, Ayscough KR, Zoladek T., Amino acid substitution equivalent to human chorea-acanthocytosis I2771R in yeast Vps13 protein affects its binding to phosphatidylinositol 3-phosphate, Hum Mol Genet, 2017, 04/15, 26, 8, 1497-1510
  • Kaminska J, Rzepnikowska W, Polak A, Flis K, Soczewka P, Bala K, Sienko M, Grynberg M, Kaliszewski P, Urbanek A, Ayscough K, Zoladek T., Phosphatidylinositol-3-phosphate regulates response of cells to proteotoxic stress, Int J Biochem Cell Biol, 2016, 10, 79, 494-504
  • Ellen G. Allwood, Joe J. Tyler, Agnieszka N. Urbanek, Iwona I. Smaczynska-de Rooij, and Kathryn R. Ayscough., Elucidating Key Motifs Required for Arp2/3-Dependent and Independent Actin Nucleation by Las17/WASP, PLoS One, 2016, 11, 9, e0163177
  • Weronika Rzepnikowska, Joanna Kaminska, Agnieszka Urbanek, Iwona Smaczynska de-Rojii, Kathryn Ayscough, Teresa Zoladek., Vps13 protein is involved in endocytic internalization and endosomal trafficking events in yeast, Conference paper, 09/2015, 27th International Conference on Yeast Genetics and Molecular Biology, Levico Terme, Trentino, Italy, 32
  • Urbanek AN, et al., Distinct actin and lipid binding sites in Ysc84 are required during early stages of yeast endocytosis, PLoS One, 2015, 08/27, 10, 8, e0136732
  • Urbanek AN, et al., Function and interactions of the Ysc84/SH3yl1 family of actin- and lipid-binding proteins, BST, 2015, 43, 1, 111-116
  • Urbanek AN, et al., A novel actin-binding motif in Las17/WASP nucleates actin filaments independently of Arp2/3, Curr Biol, 2013, 23, 3, 196-203

Communications and Presentations

2025 - NeuroBioUK – poster presentation

2025 – ARUK Northern Alliance – oral presentation

2025 – ADPD 2025 – poster presentation

2023 – NECB-North of England Cell Biology meeting– poster presentation

2014, oral presentation, 30 October, University of Manchester, Cell-Matrix interaction centre, an invited speaker

2014, poster presentation, 12 September, NECB-North of England Cell Biology meeting, Leeds

2014, selected oral presentation, 4-7 September, Cambridge, Dynamic Cell  2013, oral presentation, 06 December, Bristol, UK, Actin conference.

2013, oral presentation, CMIAD, University of Sheffield

2011, poster presentation, 16 December, Bristol, UK, Actin conference.

2011, poster presentation, 29 October–02 November, Stresa-Lake Maggiore, Italy, Actin-Based Motility - From Molecules to Model Organisms.

2011, poster presentation, 3-5 April, Edinburgh, UK, Molecular Mechanisms of Exocytosis and Endocytosis

2009, poster presentation, 11 December, Bristol, UK, Actin conference

• The University of Sheffield, Membrane trafficking group meetings since 2008

Professional Skills

• FHEA – Fellow of the Higher Education Academy.

• Project management, including staff, budget and priority setting.

• Complex problem solving, critical thinking and troubleshooting.

•  Skilled in training, managing, supervising and motivating people at different levels

• Mentor at Thesis mentoring programme.

• Science communication, public engagement at primary and secondary school levels, member of the Faculty of Medicine, Dentistry &Health Research Staff Association (MDHRSA): communication team.

• Mentor at Thesis mentoring programme.

Timeline

Postdoctoral Research Associate

University of Sheffield
01.2023 - 09.2025

Research Assistant

University of Sheffield
01.2020 - 01.2023

Senior Research Technician

University of Sheffield
01.2017 - 01.2019

Postdoctoral Research Associate

University of York
01.2015 - 01.2017

Postdoctoral Research Associate

University of Sheffield
01.2012 - 01.2015

PhD - Cell Biology

University of Sheffield
01.2008 - 01.2012

Master's and an engineer - undefined

West Pomeranian University of Technology (the University of Agriculture)
01.2001 - 01.2006
Agnieszka N. Urbanek