Summary
Overview
Work History
Education
Skills
Accomplishments
Software
Interests
Publications and Conference Presentations
Professional Development
Conferences and Presentations
Timeline
Generic

Ashish Bhandari

Preston,LAN

Summary

Experienced Graduate Teaching Assistant with MS in Biological sciences and demonstrated history of working in academia and research. Passionate about microscopy and experienced in developmental biology, enhanced with skills in patch clamp electrophysiology, optogenetics, optic neurodegeneration models, and an extensive experience in cell culture, and microbiological techniques. Research goals focused on understanding plasticity in the visual system in response to injury and disease.

Overview

9
9
years of professional experience
14
14
years of post-secondary education
3
3
Languages

Work History

Graduate Research Assistant

University of Nebraska Medical Center
08.2017 - 01.2022
  • Extensive experience in microscopy and single cell patch clamp electrophysiology from retina, brain slices, and cell culture
  • Published peer reviewed work in understanding structural and functional plasticity in the visual thalamus in response to induced retinal injury in mouse models of Glaucoma (microbead injections to the anterior chamber, and enucleation models)
  • Developed and optimized protocols for analyzing single cell fills and microscopy images obtained using 2-photon and fluorescence imaging.
  • Completed administrative and research duties per professor request.
  • Gathered, reviewed, and summarized literature from scientific journals such as SciFinder and PubMed and produced graphs and other scientific calculations using MS Excel.
  • Maintained and calibrated equipment for precision and accuracy.
  • Planned, modified and executed research techniques, procedures and tests.

Graduate Teaching Assistant

University of Houston
08.2015 - 08.2017
  • Utilized cell culture experience in preparing materials for genetics, and Cell Biology labs for undergraduates.
  • Prepared and maintained drosophila stocks, culture, and propagation of mammalian cell lines as well as protozoal culture
  • Managed undergraduate labs for Genetics, and Cell Biology.
  • Checked assignments, proctored tests, and provided grades according to university standards.
  • Oversaw classes of up to 30 students in Introduction to Biological Sciences, Anatomy and Physiology, Genetics, and Cell Biology.
  • Worked with teachers in evaluating student progress, needs and gains.
  • Led courses independently with minimal oversight from professors.
  • Applied fair grading practices consistently to evaluate students' work and assign letter or number values.
  • Copied, organized and distributed classroom materials.

Graduate Research Assistant

University of Houston
06.2016 - 08.2016
  • Evaluated gene expression in Xenopus laevis tadpole brains for indications of inflammatory response induced by brain injury using quantitative PCR and in situ hybridization
  • Immunofluorescence staining and confocal imaging of Xenopus tadpole brain samples for the expression of different protein markers in response to brain injury
  • Helped devise an experimental device to induce focal brain injury in X. laevis tadpoles.
  • Completed administrative and research duties per professor request.
  • Planned, modified, and executed research techniques, procedures and tests.
  • Gathered, reviewed, and summarized literature from scientific journals such as SciFinder and PubMed and produced graphs and other scientific calculations using MS Excel.
  • Maintained and calibrated equipment for precision and accuracy.

Rotation Student

University of Houston
01.2016 - 05.2016
  • Prepared, and imaged the Dentate Gyrus in a mouse model of Dravet Syndrome for Astrocyte specific marker GFAP to quantify Astrocyte activation and density utilizing Farsight software package.

Research Assistant

Annapurna Neurological Institute and Allied Sciences
08.2014 - 01.2015
  • Optimized PCR protocols for routine Tuberculosis diagnosis from patient samples increasing result accuracy and turnout period
  • Managed procuring lab supplies and organizing day to day activities in the lab.

Joint Treasurer

Youth Initiative
08.2010 - 08.2012
  • Carried out day-to-day duties accurately and efficiently.
  • Participated in team-building activities to enhance working relationships..
  • Resolved problems, improved operations and provided exceptional service.

Education

Graduate Coursework - Neuroscience

University of Nebraska Medical Center
Omaha, Nebraska, USA
08.2017 - 01.2022

Master of Science (M.S) - Biology/Biological Sciences

University of Houston
Houston, Texas, USA
08.2015 - 08.2017

Bachelor of Science (BSc) - Biotechnology, Biology, General

SANN International College
Kathmandu, Nepal
09.2009 - 12.2014

High School Diploma -

Nobel Academy Higher Secondary School
Kathmandu, Nepal
06.2006 - 06.2008

Skills

Patch Clamp ElectrophysiologyConfocal and Multi-photon/2-photon MicroscopyMolecular biologyCell fills, dendritic reconstruction, and Sholl Analysis

Scientific paper experience

Experiment development

Research and analysis

Project Management

Research reporting

Technical Writing

Lab work

Experimental design

Grant writing

Running experiments

Lab safety

Data analysis

Academic research

Data organization

Lab operations

Database searches

Academic presentations

Accomplishments

  • UNMC Assistantships/FeIIowships - University of Nebraska Medical Center - 2020. 2-year Graduate Fellowship based on internal peer reviewed research proposal.
  • Travel Award and Scholarship - Jackson Laboratory. Travel award scholarship to attend 'The Whole Scientist 2019' in Jackson Laboratory, Maine, USA.
  • Travel Award and Scholarship - The Jackson Laboratory. Travel award to attend The Whole Scientist 2018 at Jackson Laboratory, Maine, USA.
  • Presidential Graduate Fellowship - University of Houston Aug 2015 - Aug 2017. 2-year fellowship awarded to students with outstanding achievements evaluated by the admissions committee.
  • Collegiate Ambassador for Peace - Universal Peace Federation and World Collegiate Association for the Research of Principles - 2015.
  • Merit based Undergraduate Tuition Scholarship - SANN International College - 2013.

Software

ImageJ

GraphPad Prism

Clampfit

Adobe

LaTeX

Farsight

Microsoft Office

Linux

MacOS

Windows OS

Interests

2-Photon and Multiphoton Microscopy

Confocal Microscopy

Optical Microscopy

Cell Culture

Protozoan Culture

Patch-clamp Electrophysiology

Molecular Biology

Single cell imaging and Structural analysis

Publications and Conference Presentations

Publications

  • “Structural and Functional Plasticity in the Dorsolateral Geniculate Nucleus of Mice following Bilateral Enucleation”, Neuroscience, 4 February 2022. https://doi.org/10.1016/j.neuroscience.2022.01.029
  • “Early-Stage Ocular Hypertension Alters Retinal Ganglion Cell Synaptic Transmission in the Visual Thalamus”, Frontiers in Cellular Neuroscience, 19th September 2019. https://doi.org/10.3389/fncel.2019.00426

Professional Development

  • The Whole Scientist - Jackson Laboratory, Maine, USA, May 2019.
  • UNMC International Student Ambassador - University of Nebraska Medical Center, Nebraska, USA, 2019.
  • The Whole Scientist - Jackson Laboratory, Maine, USA, May 2018.
  • Student Leadership Camp: Student Seminar Towards Responsible Leadership - Youth Initiative, Kathmandu, Nepal, August 2008.
  • General Member - Youth Initiative, Kathmandu, Nepal, 2008 - 2012.
  • Civic Leadership School - Youth Initiative, Kathmandu, Nepal, December 2009.
  • Joint Treasurer - Youth Initiative, Kathmandu, Nepal, 2010 - 2012.
  • Trainer of Trainers (TOT): Life Skills Development Program - Youth Initiative, Kathmandu, Nepal, June 2011.
  • General Member - American Association for the Advancement of Science, 2015

Conferences and Presentations

  • Conference Talk - Bhandari, A.; Smith, J. , Van Hook, Matthew J. , "Bilateral enucleations alters the intrinsic excitability of thalamocortical neurons in the dLGN", University of Nebraska Medical Center, Jun 2020.
  • Conference Talk - Bhandari, A.; Smith, J., Van Hook, Matthew J., "Bilateral enucleations alters the intrinsic excitability of thalamocortical neurons in the dLGN", Biotechnology Society of Nepal (BSN), Jun 2020.
  • Conference Poster - Bhandari, A.: Smith, J., Van Hook, Matthew J., "Bilateral enucleations alters the intrinsic excitability of thalamocortical neurons in the dLGN", The University of Iowa Medical Education and Research Facility, Aug 2019.
  • Conference Poster - Bhandari, A.; Smith, J., Van Hook, Matthew J., "Bilateral enucleations alters the intrinsic excitability of thalamocortical neurons in the dLGN", University of Nebraska Medical Center, Jun 2019.
  • Conference Poster - Bhandari, A.; Smith, J., Van Hook, Matthew J., "Bilateral enucleations alters the intrinsic excitability of thalamocortical neurons in the dLGN", Association for Research in Vision and Ophthalmology (ARVO), April 2019.
  • Conference Poster - Bhandari, A.; Smith, J., Van Hook, Matthew J., "Inhibitory and Excitatory Inputs to Alpha Retinal Ganglion Cells are Unaffected in a Mouse Model of Ocular Hypertension”, The University of Iowa Medical Education and Research Facility, Aug 2018.
  • Conference Poster – “Molecular Characterization of in-vitro propagated orchids using RAPD”, International Conference on Biotechnology – Nepal, Jan 2015.
  • Conference Poster – “Bioactivity of phytochemical extracts from Taxus wallichiana Zucc.Conference Poster - Bioactivity of phytochemical extracts from Taxus wallichiana Zucc.”, National Conference on Biotechnology – Nepal, Jan 2014.
  • Conference Poster - Bhandari, A.; Lamichhane, N.; Lamichhane, S.; Shakya, P., "An efficient method for genomic DNA extraction from silica dried leaves of Taxus sps", National Conference on Biotechnology – Nepal, Jan 2013.

Timeline

Graduate Coursework - Neuroscience

University of Nebraska Medical Center
08.2017 - 01.2022

Graduate Research Assistant

University of Nebraska Medical Center
08.2017 - 01.2022

Graduate Research Assistant

University of Houston
06.2016 - 08.2016

Rotation Student

University of Houston
01.2016 - 05.2016

Master of Science (M.S) - Biology/Biological Sciences

University of Houston
08.2015 - 08.2017

Graduate Teaching Assistant

University of Houston
08.2015 - 08.2017

Research Assistant

Annapurna Neurological Institute and Allied Sciences
08.2014 - 01.2015

Joint Treasurer

Youth Initiative
08.2010 - 08.2012

Bachelor of Science (BSc) - Biotechnology, Biology, General

SANN International College
09.2009 - 12.2014

High School Diploma -

Nobel Academy Higher Secondary School
06.2006 - 06.2008
Ashish Bhandari